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Literature summary for 3.5.1.28 extracted from

  • Litzinger, S.; Duckworth, A.; Nitzsche, K.; Risinger, C.; Wittmann, V.; Mayer, C.
    Muropeptide rescue in Bacillus subtilis involves sequential hydrolysis by beta-N-acetylglucosaminidase and N-acetylmuramyl-L-alanine amidase (2010), J. Bacteriol., 192, 3132-3143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells as a cytoplasmic N-terminal His10-tagged fusion protein Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end ?
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?
additional information Bacillus subtilis 168 AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end ?
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?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography, gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end Bacillus subtilis ?
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?
additional information AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end Bacillus subtilis 168 ?
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?
N-acetylmuramoyl-L-alanine + H2O
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Bacillus subtilis N-acetylmuramate + L-alanine
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?
N-acetylmuramoyl-L-alanine + H2O
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Bacillus subtilis 168 N-acetylmuramate + L-alanine
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?

Synonyms

Synonyms Comment Organism
AmiE formerly YbbE Bacillus subtilis
N-acetylmuramyl-L-alanine amidase
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Bacillus subtilis

General Information

General Information Comment Organism
metabolism AmiE and NagZ in conjunction liberate MurNAc by sequential hydrolysis of muropeptides Bacillus subtilis