Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells as a cytoplasmic N-terminal His10-tagged fusion protein | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end | ? | - |
? | |
additional information | Bacillus subtilis 168 | AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography, gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end | Bacillus subtilis | ? | - |
? | |
additional information | AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end | Bacillus subtilis 168 | ? | - |
? | |
N-acetylmuramoyl-L-alanine + H2O | - |
Bacillus subtilis | N-acetylmuramate + L-alanine | - |
? | |
N-acetylmuramoyl-L-alanine + H2O | - |
Bacillus subtilis 168 | N-acetylmuramate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmiE | formerly YbbE | Bacillus subtilis |
N-acetylmuramyl-L-alanine amidase | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | AmiE and NagZ in conjunction liberate MurNAc by sequential hydrolysis of muropeptides | Bacillus subtilis |