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Literature summary for 3.5.1.28 extracted from

  • Kajimura, J.; Fujiwara, T.; Yamada, S.; Suzawa, Y.; Nishida, T.; Oyamada, Y.; Hayashi, I.; Yamagishi, J.; Komatsuzawa, H.; Sugai, M.
    Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus (2005), Mol. Microbiol., 58, 1087-1101.
    View publication on PubMed
Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment) Organism
gene sle1, DNA and amino acid sequence determination and analysis, expression of His6-tagged 128 C-terminal residues and of His6-tagged 180 N-terminal residues in Escherichia coli strain XL-1I Blue Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
additional information an insertion mutation of gene sle1 leads to impaired cell separation and induced cluster formation of the cells, the mutant cells are less pathogenic than the wild-type cells in mice, phenotype, overview Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Staphylococcus aureus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, processed enzyme, SDS-PAGE x * 35800, unprocessed enzyme, sequence calculation Staphylococcus aureus
35800
-
 x * 32000, processed enzyme, SDS-PAGE x * 35800, unprocessed enzyme, sequence calculation Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus aureus the enzyme performs cell wall lysis and is important in cell separation of Staphylococcus aureus ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme possesses a signal peptide for secretion from the cell Staphylococcus aureus

Purification (Commentary)

Purification (Comment) Organism
native enzyme 307.7fold from cell culture medium by dye-ligand affinity chromatography, the flow through of the step is used in hydrophobic interaction chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme performs cell wall lysis and is important in cell separation of Staphylococcus aureus Staphylococcus aureus ?
-
 ?
additional information the activity of recombinant C-terminal and N-terminal enzyme fragments towards cell wall peptidoglycans is analyzed by MALDI-TOF MS Staphylococcus aureus ?
-
 ?
N-acetylmuramoyl-L-alanine + H2O the enzyme performs cell wall lysis by cleavage of N-acetylmuramyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan Staphylococcus aureus N-acetylmuramate + L-alanine
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, processed enzyme, SDS-PAGE x * 35800, unprocessed enzyme, sequence calculation Staphylococcus aureus

Synonyms

Synonyms Comment Organism
Sle1
-
 Staphylococcus aureus

pI Value

Organism Comment pI Value Maximum pI Value
Staphylococcus aureus sequence calculation
-
 9.8