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Literature summary for 3.5.1.28 extracted from

  • Kleppe, G.; Jensen, H.B.; Pryme, J.F.
    Purification and characterization of the lytic enzyme N-acetylmuramyl-L-alanine amidase of bacteriophage T7 (1977), Eur. J. Biochem., 76, 317-326.
    View publication on PubMed

General Stability

General Stability Organism
very instable Escherichia phage T7

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates enzyme at concentrations up to 1 mM Escherichia phage T7

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
PAGE Escherichia phage T7
21000
-
gel filtration Escherichia phage T7

Organism

Organism UniProt Comment Textmining
Escherichia phage T7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
840fold Escherichia phage T7

Storage Stability

Storage Stability Organism
0°C, activity decreases by 97% in the first 24 hours after preparation Escherichia phage T7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Escherichia phage T7