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Literature summary for 3.5.1.25 extracted from

  • Yamano, N.; Higashida, N.; Endo, C.; Sakata, N.; Fujishima, S.; Maruyama, A.; Higashihara, T.
    Purification and characterization of N-acetylglucosamine-6-phosphate deacetylase from a psychrotrophic marine bacterium, Alteromonas species (2000), Mar. Biotechnol., 2, 57-64.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Alteromonas sp.
Cu2+
-
Alteromonas sp.
Cu2+
-
Vibrio cholerae serotype O1
EDTA
-
Alteromonas sp.
EDTA
-
Vibrio cholerae serotype O1
iodoacetate
-
Alteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
N-acetylglucosamine 6-phosphate
-
Vibrio cholerae serotype O1
0.3
-
N-acetyl-D-glucosamine 6-phosphate at 10°C Alteromonas sp.
0.38
-
N-acetylglucosamine 6-phosphate at 30°C Alteromonas sp.
4.6
-
N-acetylglucosamine 6-sulfate
-
Vibrio cholerae serotype O1
5.9
-
N-acetylglucosamine 6-sulfate at 10°C Alteromonas sp.
12
-
N-acetylglucosamine 6-sulfate at 30°C Alteromonas sp.
73
-
N-acetylglucosamine
-
Vibrio cholerae serotype O1
98
-
N-acetylglucosamine at 10°C Alteromonas sp.
120
-
N-acetylglucosamine at 30°C Alteromonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Vibrio cholerae serotype O1
Co2+ 270% increase of activity by 5 mM Alteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
148000
-
gel filtration Alteromonas sp.
164000
-
non-denaturing PAGE Alteromonas sp.

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
-
-
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Alteromonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
-
Alteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine 6-phosphate + H2O
-
Vibrio cholerae serotype O1 D-glucosamine 6-phosphate + acetate
-
?
N-acetyl-D-glucosamine 6-phosphate + H2O
-
Alteromonas sp. D-glucosamine 6-phosphate + acetate
-
?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
Vibrio cholerae serotype O1 D-glucosamine 6-sulfate + acetate
-
?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
Alteromonas sp. D-glucosamine 6-sulfate + acetate
-
?
N-acetylglucosamine + H2O
-
Vibrio cholerae serotype O1 D-glucosamine + acetate
-
?
N-acetylglucosamine + H2O
-
Alteromonas sp. D-glucosamine + acetate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
hydrolysis of N-acetylglucosamine Alteromonas sp.
55
-
hydrolysis of N-acetylglucosamine 6-phosphate Alteromonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 about 40% of maximal activity at 20°C and at 60°C, hydrolysis of N-acetylglucosamine Alteromonas sp.
40 70 40°C: about 50% of maximal activity, 65°C: 60% of maximal activity, 70°C: 14% of maximal activity, hydrolysis of N-acetylglucosamine 6-phosphate Alteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
30 min, stable up to Alteromonas sp.
60
-
30 min, complete inactivation Alteromonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
N-acetylglucosamine at 10°C Alteromonas sp.
0.074
-
N-acetylglucosamine 6-sulfate at 30°C Alteromonas sp.
0.12
-
N-acetylglucosamine 6-phosphate at 10°C Alteromonas sp.
0.34
-
N-acetylglucosamine 6-sulfate at 30°C Alteromonas sp.
0.63
-
N-acetylglucosamine at 30°C Alteromonas sp.
0.68
-
N-acetylglucosamine 6-phosphate at 30°C Alteromonas sp.
0.97
-
N-acetylglucosamine 6-phosphate
-
Vibrio cholerae serotype O1
25
-
N-acetylglucosamine
-
Vibrio cholerae serotype O1
50
-
N-acetylglucosamine 6-sulfate
-
Vibrio cholerae serotype O1