Application | Comment | Organism |
---|---|---|
medicine | bile salt hydrolase (BSH) active probiotic strains are being used in the treatment of hypercholesterolemia related diseases | Lactobacillus gasseri |
Cloned (Comment) | Organism |
---|---|
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL1-Blue | Lactobacillus gasseri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycochenodeoxycholic acid + H2O | Lactobacillus gasseri | - |
chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | Lactobacillus gasseri ATCC 33323 | - |
chenodeoxycholate + glycine | - |
? | |
glycocholic acid + H2O | Lactobacillus gasseri | - |
cholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | Lactobacillus gasseri | - |
deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | Lactobacillus gasseri ATCC 33323 | - |
deoxycholate + glycine | - |
? | |
taurocholic acid + H2O | Lactobacillus gasseri | - |
cholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | Lactobacillus gasseri | - |
deoxycholate + taurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus gasseri | A0A1V0FWZ3 | isolated from human gut or faeces | - |
Lactobacillus gasseri ATCC 33323 | A0A1V0FWZ3 | isolated from human gut or faeces | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography, partially | Lactobacillus gasseri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycochenodeoxycholic acid + H2O | - |
Lactobacillus gasseri | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | moderate activity | Lactobacillus gasseri | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | - |
Lactobacillus gasseri ATCC 33323 | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | moderate activity | Lactobacillus gasseri ATCC 33323 | chenodeoxycholate + glycine | - |
? | |
glycocholic acid + H2O | - |
Lactobacillus gasseri | cholate + glycine | - |
? | |
glycocholic acid + H2O | high activity | Lactobacillus gasseri | cholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | - |
Lactobacillus gasseri | deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | best substrate | Lactobacillus gasseri | deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | - |
Lactobacillus gasseri ATCC 33323 | deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | best substrate | Lactobacillus gasseri ATCC 33323 | deoxycholate + glycine | - |
? | |
additional information | substrate specificity of BSH from Lactobacillus gasseri strain ATCC 33323, the enzyme shows a preference for the glycine-conjugated bile salts. No activity with taurochenodeoxycholic acid | Lactobacillus gasseri | ? | - |
? | |
additional information | substrate specificity of BSH from Lactobacillus gasseri strain ATCC 33323, the enzyme shows a preference for the glycine-conjugated bile salts. No activity with taurochenodeoxycholic acid | Lactobacillus gasseri ATCC 33323 | ? | - |
? | |
taurocholic acid + H2O | - |
Lactobacillus gasseri | cholate + taurine | - |
? | |
taurocholic acid + H2O | low activity | Lactobacillus gasseri | cholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | - |
Lactobacillus gasseri | deoxycholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | very low activity | Lactobacillus gasseri | deoxycholate + taurine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Lactobacillus gasseri |
BSH | - |
Lactobacillus gasseri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Lactobacillus gasseri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 95 | activity range, profile overview | Lactobacillus gasseri |
37 | 65 | over 50% of maximal activity within this range | Lactobacillus gasseri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
recombinant enzyme | Lactobacillus gasseri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | activity range, profile overview | Lactobacillus gasseri |
General Information | Comment | Organism |
---|---|---|
physiological function | mechanism of action of the BSH of probiotics against hypercholesterolemia related diseases | Lactobacillus gasseri |