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Literature summary for 3.5.1.24 extracted from

  • Ba, N.; Oeztuerk, M.
    Molecular cloning and characterization of bile salt hydrolase from Lactobacillus gasseri ATCC 33323 strain (2018), Food Biotechnol., 32, 95-111 .
No PubMed abstract available

Application

Application Comment Organism
medicine bile salt hydrolase (BSH) active probiotic strains are being used in the treatment of hypercholesterolemia related diseases Lactobacillus gasseri

Cloned(Commentary)

Cloned (Comment) Organism
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL1-Blue Lactobacillus gasseri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycochenodeoxycholic acid + H2O Lactobacillus gasseri
-
chenodeoxycholate + glycine
-
?
glycochenodeoxycholic acid + H2O Lactobacillus gasseri ATCC 33323
-
chenodeoxycholate + glycine
-
?
glycocholic acid + H2O Lactobacillus gasseri
-
cholate + glycine
-
?
glycodeoxycholic acid + H2O Lactobacillus gasseri
-
deoxycholate + glycine
-
?
glycodeoxycholic acid + H2O Lactobacillus gasseri ATCC 33323
-
deoxycholate + glycine
-
?
taurocholic acid + H2O Lactobacillus gasseri
-
cholate + taurine
-
?
taurodeoxycholic acid + H2O Lactobacillus gasseri
-
deoxycholate + taurine
-
?

Organism

Organism UniProt Comment Textmining
Lactobacillus gasseri A0A1V0FWZ3 isolated from human gut or faeces
-
Lactobacillus gasseri ATCC 33323 A0A1V0FWZ3 isolated from human gut or faeces
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography, partially Lactobacillus gasseri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycochenodeoxycholic acid + H2O
-
Lactobacillus gasseri chenodeoxycholate + glycine
-
?
glycochenodeoxycholic acid + H2O moderate activity Lactobacillus gasseri chenodeoxycholate + glycine
-
?
glycochenodeoxycholic acid + H2O
-
Lactobacillus gasseri ATCC 33323 chenodeoxycholate + glycine
-
?
glycochenodeoxycholic acid + H2O moderate activity Lactobacillus gasseri ATCC 33323 chenodeoxycholate + glycine
-
?
glycocholic acid + H2O
-
Lactobacillus gasseri cholate + glycine
-
?
glycocholic acid + H2O high activity Lactobacillus gasseri cholate + glycine
-
?
glycodeoxycholic acid + H2O
-
Lactobacillus gasseri deoxycholate + glycine
-
?
glycodeoxycholic acid + H2O best substrate Lactobacillus gasseri deoxycholate + glycine
-
?
glycodeoxycholic acid + H2O
-
Lactobacillus gasseri ATCC 33323 deoxycholate + glycine
-
?
glycodeoxycholic acid + H2O best substrate Lactobacillus gasseri ATCC 33323 deoxycholate + glycine
-
?
additional information substrate specificity of BSH from Lactobacillus gasseri strain ATCC 33323, the enzyme shows a preference for the glycine-conjugated bile salts. No activity with taurochenodeoxycholic acid Lactobacillus gasseri ?
-
?
additional information substrate specificity of BSH from Lactobacillus gasseri strain ATCC 33323, the enzyme shows a preference for the glycine-conjugated bile salts. No activity with taurochenodeoxycholic acid Lactobacillus gasseri ATCC 33323 ?
-
?
taurocholic acid + H2O
-
Lactobacillus gasseri cholate + taurine
-
?
taurocholic acid + H2O low activity Lactobacillus gasseri cholate + taurine
-
?
taurodeoxycholic acid + H2O
-
Lactobacillus gasseri deoxycholate + taurine
-
?
taurodeoxycholic acid + H2O very low activity Lactobacillus gasseri deoxycholate + taurine
-
?

Synonyms

Synonyms Comment Organism
bile salt hydrolase
-
Lactobacillus gasseri
BSH
-
Lactobacillus gasseri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Lactobacillus gasseri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 95 activity range, profile overview Lactobacillus gasseri
37 65 over 50% of maximal activity within this range Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
recombinant enzyme Lactobacillus gasseri

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8 activity range, profile overview Lactobacillus gasseri

General Information

General Information Comment Organism
physiological function mechanism of action of the BSH of probiotics against hypercholesterolemia related diseases Lactobacillus gasseri