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Literature summary for 3.5.1.24 extracted from

  • Bi, J.; Fang, F.; Lu, S.; Du, G.; Chen, J.
    New insight into the catalytic properties of bile salt hydrolase (2013), J. Mol. Catal. B, 96, 46-51.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
DTT
-
Ligilactobacillus salivarius

Cloned(Commentary)

Cloned (Comment) Organism
isozyme BSH1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) , subcloning in Escherichia coli strain JM109 Ligilactobacillus salivarius
isozyme BSH2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 Ligilactobacillus salivarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information isozymes BSH1 and BSH2 are allosteric enzymes, and show positive cooperativity, catalytic efficiency and substrate preference, but with differences, overview. Michaelis-Menten kinetics in the presence of dithiothreitol, but not in its absence. Steady-state kinetics, overview Ligilactobacillus salivarius
0.91
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT Ligilactobacillus salivarius
2.17
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT Ligilactobacillus salivarius
11.88
-
glycodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
13.57
-
taurocholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
14.12
-
glycodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
14.83
-
taurochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
15.02
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
15.04
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
15.05
-
glycocholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
15.48
-
glycocholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
15.77
-
taurodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
15.92
-
taurocholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
17.16
-
taurochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
18.12
-
taurodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, recombinant isozyme BSH1, SDS-PAGE Ligilactobacillus salivarius
36000
-
4 * 36000, recombinant isozyme BSH2, SDS-PAGE Ligilactobacillus salivarius
140000
-
recombinant isozyme BSH1, gel filtration Ligilactobacillus salivarius
142000
-
recombinant isozyme BSH2, gel filtration Ligilactobacillus salivarius

Organism

Organism UniProt Comment Textmining
Ligilactobacillus salivarius
-
-
-
Ligilactobacillus salivarius LMG14476
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme BSH1 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration Ligilactobacillus salivarius
recombinant His-tagged isozyme BSH2 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration Ligilactobacillus salivarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycochenodeoxycholic acid + H2O
-
Ligilactobacillus salivarius chenodeoxycholate + glycine
-
?
glycochenodeoxycholic acid + H2O
-
Ligilactobacillus salivarius LMG14476 chenodeoxycholate + glycine
-
?
glycocholic acid + H2O
-
Ligilactobacillus salivarius cholate + glycine
-
?
glycocholic acid + H2O
-
Ligilactobacillus salivarius LMG14476 cholate + glycine
-
?
glycodeoxycholic acid + H2O
-
Ligilactobacillus salivarius deoxycholate + glycine
-
?
glycodeoxycholic acid + H2O
-
Ligilactobacillus salivarius LMG14476 deoxycholate + glycine
-
?
taurochenodeoxycholic acid + H2O
-
Ligilactobacillus salivarius chenodeoxycholate + taurine
-
?
taurochenodeoxycholic acid + H2O
-
Ligilactobacillus salivarius LMG14476 chenodeoxycholate + taurine
-
?
taurocholic acid + H2O
-
Ligilactobacillus salivarius cholate + taurine
-
?
taurodeoxycholic acid + H2O
-
Ligilactobacillus salivarius deoxycholate + taurine
-
?
taurodeoxycholic acid + H2O
-
Ligilactobacillus salivarius LMG14476 deoxycholate + taurine
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 36000, recombinant isozyme BSH1, SDS-PAGE Ligilactobacillus salivarius
homotetramer 4 * 36000, recombinant isozyme BSH2, SDS-PAGE Ligilactobacillus salivarius

Synonyms

Synonyms Comment Organism
bile salt hydrolase
-
Ligilactobacillus salivarius
BSH1
-
Ligilactobacillus salivarius
BSH2
-
Ligilactobacillus salivarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ligilactobacillus salivarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.52
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT Ligilactobacillus salivarius
35.32
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT Ligilactobacillus salivarius
48.76
-
glycodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
53.82
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
70.03
-
taurocholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
70.07
-
glycocholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
80.87
-
glycodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
82.59
-
glycocholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
83.15
-
taurodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
83.77
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
91.53
-
taurochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
93.11
-
taurochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
93.14
-
taurocholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
99.43
-
taurodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 isozyme BSH1, broad optimum Ligilactobacillus salivarius
5.5 6 isozyme BSH2 Ligilactobacillus salivarius

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 isozyme BSH1, activity range, profile overview Ligilactobacillus salivarius
5.5 7.5 isozyme BSH2, activity range, profile overview Ligilactobacillus salivarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.45
-
glycodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
3.58
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
4.53
-
glycocholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
5.16
-
taurocholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
5.27
-
taurodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
5.33
-
taurochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1 Ligilactobacillus salivarius
5.49
-
glycocholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
5.49
-
taurodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
5.57
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
5.85
-
taurocholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
6.28
-
taurochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
6.81
-
glycodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2 Ligilactobacillus salivarius
16.82
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT Ligilactobacillus salivarius
36.84
-
glycochenodeoxycholic acid pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT Ligilactobacillus salivarius