Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Ligilactobacillus salivarius |
Cloned (Comment) | Organism |
---|---|
isozyme BSH1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) , subcloning in Escherichia coli strain JM109 | Ligilactobacillus salivarius |
isozyme BSH2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Ligilactobacillus salivarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | isozymes BSH1 and BSH2 are allosteric enzymes, and show positive cooperativity, catalytic efficiency and substrate preference, but with differences, overview. Michaelis-Menten kinetics in the presence of dithiothreitol, but not in its absence. Steady-state kinetics, overview | Ligilactobacillus salivarius | |
0.91 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT | Ligilactobacillus salivarius | |
2.17 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT | Ligilactobacillus salivarius | |
11.88 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
13.57 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
14.12 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
14.83 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
15.02 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
15.04 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
15.05 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
15.48 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
15.77 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
15.92 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
17.16 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
18.12 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, recombinant isozyme BSH1, SDS-PAGE | Ligilactobacillus salivarius |
36000 | - |
4 * 36000, recombinant isozyme BSH2, SDS-PAGE | Ligilactobacillus salivarius |
140000 | - |
recombinant isozyme BSH1, gel filtration | Ligilactobacillus salivarius |
142000 | - |
recombinant isozyme BSH2, gel filtration | Ligilactobacillus salivarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ligilactobacillus salivarius | - |
- |
- |
Ligilactobacillus salivarius LMG14476 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme BSH1 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration | Ligilactobacillus salivarius |
recombinant His-tagged isozyme BSH2 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration | Ligilactobacillus salivarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | chenodeoxycholate + glycine | - |
? | |
glycocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + glycine | - |
? | |
glycocholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | cholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | deoxycholate + glycine | - |
? | |
taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | chenodeoxycholate + taurine | - |
? | |
taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | chenodeoxycholate + taurine | - |
? | |
taurocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | deoxycholate + taurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36000, recombinant isozyme BSH1, SDS-PAGE | Ligilactobacillus salivarius |
homotetramer | 4 * 36000, recombinant isozyme BSH2, SDS-PAGE | Ligilactobacillus salivarius |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Ligilactobacillus salivarius |
BSH1 | - |
Ligilactobacillus salivarius |
BSH2 | - |
Ligilactobacillus salivarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ligilactobacillus salivarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.52 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT | Ligilactobacillus salivarius | |
35.32 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT | Ligilactobacillus salivarius | |
48.76 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
53.82 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
70.03 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
70.07 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
80.87 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
82.59 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
83.15 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
83.77 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
91.53 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
93.11 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
93.14 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
99.43 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | isozyme BSH1, broad optimum | Ligilactobacillus salivarius |
5.5 | 6 | isozyme BSH2 | Ligilactobacillus salivarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | isozyme BSH1, activity range, profile overview | Ligilactobacillus salivarius |
5.5 | 7.5 | isozyme BSH2, activity range, profile overview | Ligilactobacillus salivarius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.45 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
3.58 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
4.53 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
5.16 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
5.27 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
5.33 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
5.49 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
5.49 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
5.57 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
5.85 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
6.28 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
6.81 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
16.82 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT | Ligilactobacillus salivarius | |
36.84 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT | Ligilactobacillus salivarius |