Application | Comment | Organism |
---|---|---|
food industry | enzyme inhibitors are promising feed additives to replace antibiotic growth promoters for enhancing the productivity and sustainability of food animals | Ligilactobacillus salivarius |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) clone (JL 885) | Ligilactobacillus salivarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 22.4% inhibition at 5 mM | Ligilactobacillus salivarius | |
CoCl2 | 95.9% inhibition at 5 mM | Ligilactobacillus salivarius | |
CuCl2 | 98.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
CuSO4 | 91.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
FeCl3 | 73% inhibition at 5 mM | Ligilactobacillus salivarius | |
FeSO4 | 96.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
KCl | 25.9% inhibition at 5 mM | Ligilactobacillus salivarius | |
KI | 36.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
KIO3 | 96.5% inhibition at 5 mM | Ligilactobacillus salivarius | |
MgCl2 | 25.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
MgSO4 | 31.3% inhibition at 5 mM | Ligilactobacillus salivarius | |
MnCl2 | 68.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
MnSO4 | 83.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
additional information | no inhibition by citric acid | Ligilactobacillus salivarius | |
Na2SeO3 | 93.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
Na2SO4 | 27.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
NaCl | 27.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
NaHCO3 | 20.6% inhibition at 5 mM | Ligilactobacillus salivarius | |
NaI | 31.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
NaIO3 | 86.4% inhibition at 5 mM | Ligilactobacillus salivarius | |
NaIO4 | 96.4% inhibition at 5 mM | Ligilactobacillus salivarius | |
vitamin C | 21.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
ZnCl2 | 68.3% inhibition at 5 mM | Ligilactobacillus salivarius | |
ZnSO4 | 89.5% inhibition at 5 mM | Ligilactobacillus salivarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.15 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
1.71 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
2.48 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
2.5 | 3 | taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
3.19 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
3.21 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycochenodeoxycholic acid + H2O | Ligilactobacillus salivarius | best substrate | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | best substrate | chenodeoxycholate + glycine | - |
? | |
glycocholic acid + H2O | Ligilactobacillus salivarius | - |
cholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
deoxycholate + glycine | - |
? | |
taurochenodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
chenodeoxycholate + taurine | - |
? | |
taurochenodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
chenodeoxycholate + taurine | - |
? | |
taurocholic acid + H2O | Ligilactobacillus salivarius | - |
cholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
deoxycholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
deoxycholate + taurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ligilactobacillus salivarius | J7H3P9 | isolated from chicken | - |
Ligilactobacillus salivarius NRRL B-30514 | J7H3P9 | isolated from chicken | - |
Purification (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme from Escherichia coli strain BL21(DE3) clone (JL 885) by nickel affinity chromatography | Ligilactobacillus salivarius |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycodeoxycholic acid | Ligilactobacillus salivarius |
5.6 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurocholic acid | Ligilactobacillus salivarius |
7.7 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycocholic acid | Ligilactobacillus salivarius |
8 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurochenodeoxycholic acid | Ligilactobacillus salivarius |
8.5 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurodeoxycholic acid | Ligilactobacillus salivarius |
17.7 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycochenodeoxycholic acid | Ligilactobacillus salivarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycochenodeoxycholic acid + H2O | best substrate | Ligilactobacillus salivarius | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | best substrate | Ligilactobacillus salivarius NRRL B-30514 | chenodeoxycholate + glycine | - |
? | |
glycocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | deoxycholate + glycine | - |
? | |
additional information | broad substrate specificity | Ligilactobacillus salivarius | ? | - |
? | |
additional information | broad substrate specificity | Ligilactobacillus salivarius NRRL B-30514 | ? | - |
? | |
taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | chenodeoxycholate + taurine | - |
? | |
taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | chenodeoxycholate + taurine | - |
? | |
taurocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + taurine | - |
? | |
taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | deoxycholate + taurine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Ligilactobacillus salivarius |
BSH | - |
Ligilactobacillus salivarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
41 | - |
recombinant enzyme | Ligilactobacillus salivarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | activity range, profile overview | Ligilactobacillus salivarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.37 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
8.5 | - |
taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
8.87 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
9.75 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
13.43 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
15.6 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
recombinant enzyme | Ligilactobacillus salivarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | activity range, profile overview | Ligilactobacillus salivarius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.04 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
3.36 | - |
taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
4.21 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
5.19 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
5.54 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
6.29 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius |