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Literature summary for 3.5.1.24 extracted from
- Purification and characterization of three different types of bile salt hydrolases from Bifidobacterium strains (2004), J. Dairy Sci., 87, 258-266.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Bifidobacterium longum subsp. infantis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium bifidum | - |
strain ATCC 11863 | - |
| Bifidobacterium longum | - |
strains ATCC 15708, KL507, and KL515 | - |
| Bifidobacterium longum subsp. infantis | - |
- |
- |
| Bifidobacterium longum subsp. infantis KL412 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 21.6fold from strain KL412 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration | Bifidobacterium longum subsp. infantis |
| native enzyme 33.5fold from strain ATCC 11863 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration | Bifidobacterium bifidum |
| native enzyme from strains ATCC 15708, KL507, and KL515 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration, 30.3fold from strain KL515 | Bifidobacterium longum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5389 | - |
purified native enzyme | Bifidobacterium longum subsp. infantis |
| 6168 | - |
purified native enzyme | Bifidobacterium bifidum |
| 7904 | - |
purified native enzyme from strain KL515 | Bifidobacterium longum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholate + H2O | - |
Bifidobacterium bifidum | glycine + chenodeoxycholate | - |
? |  |
| glycochenodeoxycholate + H2O | - |
Bifidobacterium longum subsp. infantis | glycine + chenodeoxycholate | - |
? | |
| glycochenodeoxycholate + H2O | - |
Bifidobacterium longum | glycine + chenodeoxycholate | - |
? | |
| glycochenodeoxycholate + H2O | - |
Bifidobacterium longum subsp. infantis KL412 | glycine + chenodeoxycholate | - |
? | |
| glycocholate + H2O | - |
Bifidobacterium bifidum | glycine + cholate | - |
? | |
| glycocholate + H2O | - |
Bifidobacterium longum subsp. infantis | glycine + cholate | - |
? | |
| glycocholate + H2O | - |
Bifidobacterium longum | glycine + cholate | - |
? | |
| glycocholate + H2O | - |
Bifidobacterium longum subsp. infantis KL412 | glycine + cholate | - |
? | |
| glycodeoxycholate + H2O | - |
Bifidobacterium bifidum | glycine + deoxycholate | - |
? | |
| glycodeoxycholate + H2O | - |
Bifidobacterium longum subsp. infantis | glycine + deoxycholate | - |
? | |
| glycodeoxycholate + H2O | - |
Bifidobacterium longum | glycine + deoxycholate | - |
? | |
| additional information | substrate specificity, overview | Bifidobacterium bifidum | ? | - |
? | |
| additional information | substrate specificity, overview | Bifidobacterium longum subsp. infantis | ? | - |
? | |
| additional information | substrate specificity, overview | Bifidobacterium longum | ? | - |
? | |
| additional information | substrate specificity, overview | Bifidobacterium longum subsp. infantis KL412 | ? | - |
? | |
| taurochenodeoxycholate + H2O | - |
Bifidobacterium bifidum | taurine + chenodeoxycholate | - |
? | |
| taurochenodeoxycholate + H2O | - |
Bifidobacterium longum subsp. infantis | taurine + chenodeoxycholate | - |
? | |
| taurochenodeoxycholate + H2O | - |
Bifidobacterium longum | taurine + chenodeoxycholate | - |
? | |
| taurocholate + H2O | - |
Bifidobacterium bifidum | taurine + cholate | - |
? | |
| taurocholate + H2O | - |
Bifidobacterium longum subsp. infantis | taurine + cholate | - |
? | |
| taurocholate + H2O | - |
Bifidobacterium longum | taurine + cholate | - |
? | |
| taurocholate + H2O | - |
Bifidobacterium longum subsp. infantis KL412 | taurine + cholate | - |
? | |
| taurodeoxycholate + H2O | - |
Bifidobacterium bifidum | taurine + deoxycholate | - |
? | |
| taurodeoxycholate + H2O | - |
Bifidobacterium longum subsp. infantis | taurine + deoxycholate | - |
? | |
| taurodeoxycholate + H2O | - |
Bifidobacterium longum | taurine + deoxycholate | - |
? | |
| taurodeoxycholate + H2O | - |
Bifidobacterium longum subsp. infantis KL412 | taurine + deoxycholate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Bifidobacterium longum subsp. infantis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BSH | - |
Bifidobacterium bifidum |
| BSH | - |
Bifidobacterium longum subsp. infantis |
| BSH | - |
Bifidobacterium longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bifidobacterium bifidum |
| 37 | - |
assay at | Bifidobacterium longum subsp. infantis |
| 37 | - |
assay at | Bifidobacterium longum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Bifidobacterium bifidum |
| 6.5 | - |
assay at | Bifidobacterium longum subsp. infantis |
| 6.5 | - |
assay at | Bifidobacterium longum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bifidobacterium bifidum | isoelectric focusing | - |
4.45 |
| Bifidobacterium longum subsp. infantis | isoelectric focusing | - |
4.65 |
| Bifidobacterium longum | isoelectric focusing, enzymes from strains KL507 and KL515 | - |
4.65 |
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