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Literature summary for 3.5.1.24 extracted from
- Purification and characterization of bile salt hydrolase from Bacteroides fragilis subsp. fragilis (1976), Biochim. Biophys. Acta, 452, 165-176.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
taurodeoxycholic acid | - |
Bacteroides fragilis |  |
| 0.2 | - |
glycodeoxycholic acid | - |
Bacteroides fragilis | |
| 0.26 | - |
glycochenodeoxycholic acid | - |
Bacteroides fragilis | |
| 0.29 | - |
taurochenodeoxycholic acid | - |
Bacteroides fragilis | |
| 0.35 | - |
glycocholic acid | - |
Bacteroides fragilis | |
| 0.45 | - |
taurocholic acid | - |
Bacteroides fragilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32500 | - |
x * 32500, SDS-PAGE | Bacteroides fragilis |
| 250000 | - |
gel filtration | Bacteroides fragilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | - |
ssp. fragilis ATCC 25285 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteroides fragilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160.2 | - |
- |
Bacteroides fragilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | - |
Bacteroides fragilis | 3alpha,7alpha-dihydroxy-5beta-cholanate + Gly | - |
? | |
| glycocholic acid + H2O | - |
Bacteroides fragilis | cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Bacteroides fragilis | 3alpha,12alpha-dihydroxy-5beta-cholanate + Gly | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Bacteroides fragilis | 3alpha,7alpha-dihydroxy-5beta-cholanate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Bacteroides fragilis | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Bacteroides fragilis | 3alpha,12alpha-dihydroxy-5beta-cholanate + taurine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32500, SDS-PAGE | Bacteroides fragilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Bacteroides fragilis |
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