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Literature summary for 3.5.1.23 extracted from

  • Shi, X.X.; Huang, Y.J.; Begum, M.A.; Zhu, M.F.; Li, F.Q.; Zhang, M.J.; Zhou, W.W.; Mao, C.; Zhu, Z.R.
    A neutral ceramidase, NlnCDase, is involved in the stress responses of brown planthopper, Nilaparvata lugens (Stal) (2018), Sci. Rep., 8, 1130 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.23

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
 Nilaparvata lugens

Cloned(Commentary)

Cloned (Comment) Organism
gene NlnCDase, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the enzyme in insect High Five cells in microsomes, quantitative enzyme expression analysis Nilaparvata lugens

Protein Variants

Protein Variants Comment Organism
additional information NlnCDase knockdown by specific RNA interference Nilaparvata lugens

Inhibitors

Inhibitors Comment Organism Structure
Cs+
-
 Nilaparvata lugens
EGTA
-
 Nilaparvata lugens
Fe2+
-
 Nilaparvata lugens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme has a putative signal peptide of 25 residues at the N-terminal Nilaparvata lugens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Nilaparvata lugens
additional information the enzyme activity is not affected by Mg2+, Mn2+, Ni+, and Zn2+ Nilaparvata lugens

Organism

Organism UniProt Comment Textmining
Nilaparvata lugens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Nilaparvata lugens
-
egg
-
 Nilaparvata lugens
-
head
-
 Nilaparvata lugens
-
leg
-
 Nilaparvata lugens
-
midgut
-
 Nilaparvata lugens
-
additional information the NlnCDase has higher transcript level and activity in adults than in eggs and nymphs, and in the reproductive organs (ovaries and spermaries) than in other tissues (i.e. heads, thorax, legs, midguts) Nilaparvata lugens
-
nymph
-
 Nilaparvata lugens
-
ovary
-
 Nilaparvata lugens
-
semen
-
 Nilaparvata lugens
-
thorax
-
 Nilaparvata lugens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C12:0-ceramide + H2O preferred substrate Nilaparvata lugens laurate + sphingosine
-
 ?
C16:0-ceramide + H2O low activity Nilaparvata lugens palmitate + sphingosine
-
 ?
C20:0-ceramide + H2O low activity Nilaparvata lugens arachidic acid + sphingosine
-
 ?
C24:0-ceramide + H2O low activity Nilaparvata lugens lignoceric acid + sphingosine
-
 ?
C2:0-ceramide + H2O low activity Nilaparvata lugens acetate + sphingosine
-
 ?
C6:0-ceramide + H2O
-
 Nilaparvata lugens hexanoate + sphingosine
-
 ?
additional information recombinant NlnCDase shows a broad capacity to hydrolyze different ceramide varieties and has a preference for the short and medium chain ceramide, the substrate preference in descending order is C12-ceramide, C6-ceramide, and C16-ceramide. The enzyme shows low activity for the very-short chain C2-ceramide and the long chain substrates C20-ceramide and C24-ceramide Nilaparvata lugens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80213, sequence calculation Nilaparvata lugens

Synonyms

Synonyms Comment Organism
neutral ceramidase
-
 Nilaparvata lugens
NlnCDase
-
 Nilaparvata lugens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
 recombinant enzyme Nilaparvata lugens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme Nilaparvata lugens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 NlnCDase enzyme has a broad pH range for its activity Nilaparvata lugens

pI Value

Organism Comment pI Value Maximum pI Value
Nilaparvata lugens sequence calculation
-
 5.85

Expression

Organism Comment Expression
Nilaparvata lugens transcripts and activity of the NlnCDase are upregulated under abiotic stresses including starvation, abnormal temperature, and insecticides, and biotic stress of resistant rice varieties up

General Information

General Information Comment Organism
malfunction knocking down NlnCDase by RNA interference increases female survival under starvation and temperature stresses Nilaparvata lugens
physiological function neutral ceramidase, NlnCDase, is involved in the stress responses of brown planthopper, Nilaparvata lugens. The NlnCDase level might be elevated in adult reproductive organs to mediate developmental process. NlnCDase might be involved in the stress response Nilaparvata lugens