Literature summary for 3.5.1.23 extracted from

Leclerc, J.; Garandeau, D.; Pandiani, C.; Gaudel, C.; Bille, K.; Nottet, N.; Garcia, V.; Colosetti, P.; Pagnotta, S.; Bahadoran, P.; Tondeur, G.; Mograbi, B.; Dalle, S.; Caramel, J.; Levade, T.; Ballotti, R.; Andrieu-Abadie, N.; Bertolotto, C.
Lysosomal acid ceramidase ASAH1 controls the transition between invasive and proliferative phenotype in melanoma cells (2019), Oncogene, 38, 1282-1295 .

Search for more literature for 3.5.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
gene ASAH1, quantitative RT-PCR enzyme expression analysis	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	ASAH1 silencing by siRNA. An increase in expression in the cell cycle inhibitor p27 is observed upon MITF inhibition by siRNA, but does not occur in these cells when they are transduced with ASAH1	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13510	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
501mel cell	-	Homo sapiens	-
melanoma cell	human melanoma biopsies reveal heterogeneous staining of ASAH1 and low ASAH1 expression at the melanoma invasive front. ASAH1 is highly expressed and active in about 60% of melanoma cells, and its expression correlates with that of MITF	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
acid ceramidase	-	Homo sapiens
ASAH1	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	the microphthalmia-associated transcription factor (MITF) drives expression of ASAH1. MITF is a transcription factor that binds to DNA to control the expression of its targets. MITF stimulates the activity of ASAH1 promoter	up

General Information

General Information	Comment	Organism
malfunction	ASAH1 reduction stimulates cell migration to the same extent as MITF inhibition. ASAH1 silencing in different cell types results in a significant increase in FAK phosphorylation. Conversely, forced expression of ASAH1 inhibits FAK phosphorylation. ASAH1-depleted cells are positive for the senescence-associated beta-galactosidase staining, and also MITF-silenced melanoma cells display features of senescence	Homo sapiens
metabolism	the enzyme is a target of the microphthalmia-associated transcription factor (MITF). ASAH1 controls the switch between the proliferative and invasive phenotype in melanoma cells, and MITF also is a critical regulator of switch between proliferative and invasive phenotypes promoted by melanoma plasticity. MITF is also involved in the regulation of sphingolipid metabolism	Homo sapiens
physiological function	acid ceramidase ASAH1 is a key enzyme of sphingolipid metabolism. ASAH1 controls melanoma cell proliferation and motile features. ASAH1 acts as a rheostat of the phenotypic switch in melanoma cells. Low ASAH1 expression is associated with an invasive behavior mediated by activation of the integrin alphavbeta5-FAK signaling cascade. ASAH1 controls the switch between the proliferative and invasive phenotype in melanoma cells	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)