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Literature summary for 3.5.1.23 extracted from

  • Sakamoto, W.; Coant, N.; Canals, D.; Obeid, L.M.; Hannun, Y.A.
    Functions of neutral ceramidase in the Golgi apparatus (2018), J. Lipid Res., 59, 2116-2125 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.23

Cloned(Commentary)

Cloned (Comment) Organism
cloning of human nCDase gene in pLenti6.3/TO/V5 and recombinant expression in HCT-116 cells and in cells transfected with the Golgi-targeted bacterial ceramidase and sphingomyelinase Homo sapiens

Protein Variants

Protein Variants Comment Organism
S354A site-directed mutagenesis, inactive mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
C6-urea-ceramide an nCDase inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-
additional information determination and analysis of subcellular localization of wild-type and recombinantly overexpressed immunostained nCDase Homo sapiens
-
-
plasma membrane
-
 Homo sapiens 5886
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NR71
-
-

Source Tissue

Source Tissue Comment Organism Textmining
colorectal cancer cell neutral ceramidase is highly expressed in colorectal tissues Homo sapiens
-
HCT-116 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]D-erythro-sphingosine + H2O substrate C12-NBD ceramide Homo sapiens N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoate] + D-erythro-sphingosine
-
 ?

Synonyms

Synonyms Comment Organism
nCDase
-
 Homo sapiens
neutral ceramidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction inhibition of nCDase decreases the development and progression of colorectal tumor growth. Cells overexpressing nCDase are protected from the cell death and Golgi fragmentation induced by C6-ceramide, and they show reduced levels of C6-ceramide and higher levels of sphingosine-1-phosphate (S1P) and sphingosine. nCDase-overexpressing cells show larger increases of sphingosine by 2.3fold compared to control Homo sapiens
physiological function ceramidases hydrolyze ceramides into sphingosine and fatty acids, with sphingosine being further metabolized into sphingosine-1-phosphate (S1P). Ceramidases control the levels of these bioactive sphingolipids in cells and tissues. Neutral ceramidase (nCDase) activity is involved in Wnt/beta-catenin signaling. nCDase is involved in the metabolism of C6-ceramide to sphingosine and subsequently to S1P and possibly ceramide. nCDase is found to be located in both the plasma membrane and in the Golgi apparatus, but it has minimal effects on basal levels of ceramide, sphingosine, or S1P Homo sapiens