Cloned (Comment) | Organism |
---|---|
cloning of human nCDase gene in pLenti6.3/TO/V5 and recombinant expression in HCT-116 cells and in cells transfected with the Golgi-targeted bacterial ceramidase and sphingomyelinase | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S354A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C6-urea-ceramide | an nCDase inhibitor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
additional information | determination and analysis of subcellular localization of wild-type and recombinantly overexpressed immunostained nCDase | Homo sapiens | - |
- |
plasma membrane | - |
Homo sapiens | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NR71 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colorectal cancer cell | neutral ceramidase is highly expressed in colorectal tissues | Homo sapiens | - |
HCT-116 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]D-erythro-sphingosine + H2O | substrate C12-NBD ceramide | Homo sapiens | N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoate] + D-erythro-sphingosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nCDase | - |
Homo sapiens |
neutral ceramidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of nCDase decreases the development and progression of colorectal tumor growth. Cells overexpressing nCDase are protected from the cell death and Golgi fragmentation induced by C6-ceramide, and they show reduced levels of C6-ceramide and higher levels of sphingosine-1-phosphate (S1P) and sphingosine. nCDase-overexpressing cells show larger increases of sphingosine by 2.3fold compared to control | Homo sapiens |
physiological function | ceramidases hydrolyze ceramides into sphingosine and fatty acids, with sphingosine being further metabolized into sphingosine-1-phosphate (S1P). Ceramidases control the levels of these bioactive sphingolipids in cells and tissues. Neutral ceramidase (nCDase) activity is involved in Wnt/beta-catenin signaling. nCDase is involved in the metabolism of C6-ceramide to sphingosine and subsequently to S1P and possibly ceramide. nCDase is found to be located in both the plasma membrane and in the Golgi apparatus, but it has minimal effects on basal levels of ceramide, sphingosine, or S1P | Homo sapiens |