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Literature summary for 3.5.1.23 extracted from

  • Ordonez, Y.F.; Abad, J.L.; Aseeri, M.; Casas, J.; Garcia, V.; Casasampere, M.; Schuchman, E.H.; Levade, T.; Delgado, A.; Triola, G.; Fabrias, G.
    Activity-based imaging of acid ceramidase in living cells (2019), J. Am. Chem. Soc., 141, 7736-7742 .
    View publication on PubMed
Search for more literature for 3.5.1.23

Application

Application Comment Organism
analysis development of a method for the specific visualization of catalytically active acid ceramidase in intracellular compartments is crucial for diagnosis and follow-up of therapeutic strategies in diseases linked to altered enzyme activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-bromo-N-[(2S,3R)-1,3-dihydroxynonadecan-2-yl]acetamide
-
 Homo sapiens
2-bromo-N-[(2S,3R,4E)-1,3-dihydroxyoctadec-4-en-2-yl]acetamide
-
 Homo sapiens
2-bromo-N-[(2S,3R,4Z)-1,3-dihydroxyoctadec-4-en-2-yl]acetamide
-
 Homo sapiens
2-chloro-N-[(2S,3R)-1,3-dihydroxyoctadecan-2-yl]acetamide
-
 Homo sapiens
2-chloro-N-[(2S,3R,4Z)-1,3-dihydroxyoctadec-4-en-2-yl]acetamide
-
 Homo sapiens
Bodipy-SOBRAC
-
 Homo sapiens
Bodipy-SOCLAC
-
 Homo sapiens
additional information synthesis of several analogues of SABRAC that are potent irreversible enzyme inhibitors by reaction with the active site Cys143, fluorescent SABRAC analogues are used for enzyme detection Homo sapiens
N-[(2S,3R,4E)-14-azido-1,3-dihydroxytetradec-4-en-2-yl]-2-bromoacetamide
-
 Homo sapiens
SABRAC an acid ceramidase inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13510
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-375 cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
acid ceramidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000267
-
 Bodipy-SOBRAC 25 mM sodium acetate buffer, pH 4.5, 37°C, substrate 2,4-dideoxy-2-(dodecanoylamino)-5-O-(2-oxo-2H-1-benzopyran-7-yl)-D-erythro-pentitol Homo sapiens
0.0000297
-
 2-bromo-N-[(2S,3R,4E)-1,3-dihydroxyoctadec-4-en-2-yl]acetamide 25 mM sodium acetate buffer, pH 4.5, 37°C, substrate 2,4-dideoxy-2-(dodecanoylamino)-5-O-(2-oxo-2H-1-benzopyran-7-yl)-D-erythro-pentitol Homo sapiens
0.0000357
-
 N-[(2S,3R,4E)-14-azido-1,3-dihydroxytetradec-4-en-2-yl]-2-bromoacetamide 25 mM sodium acetate buffer, pH 4.5, 37°C, substrate 2,4-dideoxy-2-(dodecanoylamino)-5-O-(2-oxo-2H-1-benzopyran-7-yl)-D-erythro-pentitol Homo sapiens
0.0000971
-
 2-chloro-N-[(2S,3R)-1,3-dihydroxyoctadecan-2-yl]acetamide 25 mM sodium acetate buffer, pH 4.5, 37°C, substrate 2,4-dideoxy-2-(dodecanoylamino)-5-O-(2-oxo-2H-1-benzopyran-7-yl)-D-erythro-pentitol Homo sapiens
0.0000988
-
 Bodipy-SOCLAC 25 mM sodium acetate buffer, pH 4.5, 37°C, substrate 2,4-dideoxy-2-(dodecanoylamino)-5-O-(2-oxo-2H-1-benzopyran-7-yl)-D-erythro-pentitol Homo sapiens

Expression

Organism Comment Expression
Homo sapiens A-375 cells are induced by doxycycline to overexpress the enzyme up

General Information

General Information Comment Organism
malfunction the enzyme is overexpressed in several types of cancer and Alzheimer's disease, and its genetic defect causes different incurable disorders Homo sapiens
additional information development of a method for the specific visualization of catalytically active acid ceramidase in intracellular compartments. Development of activity-based probes for the detection of acid ceramidase, fluorescent SABRAC enzyme inhibitor analogues are used for enzyme detection. An azide-substituted analogue allows the unprecedented labeling of active acid ceramidase in living cells. Cys143 is the catalytic residue in the active site Homo sapiens
physiological function acid ceramidase hydrolyzes ceramides into sphingoid bases and fatty acids Homo sapiens