Literature summary for 3.5.1.23 extracted from

Azuma, M.M.; Balani, P.; Boisvert, H.; Gil, M.; Egashira, K.; Yamaguchi, T.; Hasturk, H.; Duncan, M.; Kawai, T.; Movila, A.
Endogenous acid ceramidase protects epithelial cells from Porphyromonas gingivalis-induced inflammation in vitro (2018), Biochem. Biophys. Res. Commun., 495, 2383-2389 .

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of adenoviral vector expressing the human acid ceramidase (Ad-ASAH1) gene in human OBA-9 cells. ASAH1-over-expressing OBA-9 cells show significantly lower mRNA expressions of caspase-3 as well as the percentage of Annexin V-positive cells, when compared to controls. In response to stimulation with Porphyromonas gingivalis, ASAH1-overexpressing OBA-9 cells produce less TNF-alpha, IL-6, and IL1beta pro-inflammatory cytokines than observed in OBA-9 cells transduced with Ad-control vector, quantitative real-time PCR expression analysis	Homo sapiens

Cloned (Comment)

Organism

recombinant expression of adenoviral vector expressing the human acid ceramidase (Ad-ASAH1) gene in human OBA-9 cells. ASAH1-over-expressing OBA-9 cells show significantly lower mRNA expressions of caspase-3 as well as the percentage of Annexin V-positive cells, when compared to controls. In response to stimulation with Porphyromonas gingivalis, ASAH1-overexpressing OBA-9 cells produce less TNF-alpha, IL-6, and IL1beta pro-inflammatory cytokines than observed in OBA-9 cells transduced with Ad-control vector, quantitative real-time PCR expression analysis

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13510	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
epithelial cell	-	Homo sapiens	-
gingiva	a significantly lower level of acid ceramidase expression was detected in gingival tissues from periodontal patients compared to those from healthy subjects	Homo sapiens	-
additional information	no significant fluctuation is detected for neutral or alkaline ceramidases in either gingival samples or cell cultures	Homo sapiens	-
OBA-9 cell	immortalized gingival epithelial cell line	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
acid ceramidase	-	Homo sapiens
ASAH1	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	acid ceramidase expression in gingival cells is suppressed by stimulation with Porphyromonas gingivalis in vitro	down

General Information

General Information	Comment	Organism
malfunction	a significantly lower level of acid ceramidase expression was detected in gingival tissues from periodontal patients compared to those from healthy subjects	Homo sapiens
physiological function	endogenous acid ceramidase protects epithelial cells from Porphyromonas gingivalis (ATCC 33277)-induced inflammation in vitro. Anti-inflammatory and anti-apoptotic effects of acid ceramidase in host cells exposed to periodontal bacteria, and the attenuation of the expression of host-protective acid ceramidase in periodontal lesions	Homo sapiens