Literature summary for 3.5.1.23 extracted from

Zhou, Y.; Lin, X.W.; Zhang, Y.R.; Huang, Y.J.; Zhang, C.H.; Yang, Q.; Li, H.Y.; Yuan, J.Q.; Cheng, J.A.; Xu, R.; Mao, C.; Zhu, Z.R.
Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase (2013), Insect Mol. Biol., 22, 366-375.

Search for more literature for 3.5.1.23

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	infection with the rice strip virus or treatment with insecticides significantly increase LsnCer mRNA expression and its enzymatic activity in Laodelphax striatellus	Laodelphax striatellus

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, recombinant overexpression in Trichopulsia ni High Five cell membranes via baculoviral transfection, expression of GFP-tagged enzyme	Laodelphax striatellus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	20% inhibition at 5 mM	Laodelphax striatellus
Zn2+	80% inhibition at 5 mM	Laodelphax striatellus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
79300	-	x * 79300, about, sequence calculation	Laodelphax striatellus

Organism

Organism	UniProt	Comment	Textmining
Laodelphax striatellus	R4N4U2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Trichopulsia ni High Five cell membranes	Laodelphax striatellus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme has broad substrate specificity with preference for ceramides with a medium acyl-chain or a mono unsaturated long acyl-chain. The enzyme prefers ceramides with unsaturated acyl-chains over ceramides with saturated acyl-chains of the same length, and it does not use phytoceramides as substrates, suggesting that LsnCer is a ceramide-specific ceramidase	Laodelphax striatellus	?	-	?
N-arachidoyl-D-erythro-sphingosine + H2O	low activity	Laodelphax striatellus	arachidonate + D-erythro-sphingosine	-	?
N-hexanoyl-D-erythro-sphingosine + H2O	-	Laodelphax striatellus	hexanoate + D-erythro-sphingosine	-	?
N-lauroyl-D-erythro-sphingosine + H2O	best substrate	Laodelphax striatellus	laurate + D-erythro-sphingosine	-	?
N-lignoceroyl-D-erythro-sphingosine + H2O	low activity	Laodelphax striatellus	lignocerate + D-erythro-sphingosine	-	?
N-nervonoyl-D-erythro-sphingosine + H2O	-	Laodelphax striatellus	nervonoate + D-erythro-sphingosine	-	?
N-oleoyl-D-erythro-sphingosine + H2O	-	Laodelphax striatellus	oleate + D-erythro-sphingosine	-	?
N-palmitoyl-D-erythro-sphingosine + H2O	-	Laodelphax striatellus	palmitate + D-erythro-sphingosine	-	?
N-stearoyl-D-erythro-sphingosine + H2O	low activity	Laodelphax striatellus	stearate + D-erythro-sphingosine	-	?

Subunits

Subunits	Comment	Organism
?	x * 79300, about, sequence calculation	Laodelphax striatellus

Synonyms

Synonyms	Comment	Organism
LsnCer	-	Laodelphax striatellus
nCDase	-	Laodelphax striatellus
neutral ceramidase	-	Laodelphax striatellus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Laodelphax striatellus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Laodelphax striatellus

Expression

Organism	Comment	Expression
Laodelphax striatellus	infection with the rice strip virus or treatment with insecticides significantly increase LsnCer mRNA expression and its enzymatic activity in Laodelphax striatellus, quantitative real time PCR enzyme expression analysis	up

General Information

General Information	Comment	Organism
physiological function	LsnCer is a bona fide neutral ceramidase that may have a role in adaption of Laodelphax striatellus to environmental stresses	Laodelphax striatellus

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Release 2023.1 (January 2023)