Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged wild-type ACER2 and FLAG-tagged ACER2 mutants in Saccharomyces cerevisiae mutant cell microsomes, DELTAypc1 and DELTAydc1, that lack endogenous ceramidase activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ACER2 mutant, ACER2DELTAN36, lacking the N-terminal tail, the first 36 amino acid residues, exhibits undetectable activity and is mislocalized to the endoplasmic reticulum. Overexpression of ACER2, ACER2DELTAN13, but not ACER2DELTAN36 increased the release of sphingosine 1-phosphate from cells, suggesting that its mislocalization does not affect the ability of ACER2 to regulate sphingosine 1-phosphate secretion. However, overexpression of ACER2 but not ACER2DELTAN13 or ACER2DELTAN36 inhibits the glycosylation of integrin beta1 subunit and Lamp1, suggesting that its mistargeting abolishes the ability of ACER2 to regulation protein glycosylation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Homo sapiens | |
additional information | cellular ACER2 activity is inhibited by depletion of Ca2+ from Golgi lumen | Homo sapiens | |
Zn2+ | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ACER2 follows Michaelis-Menten kinetics | Homo sapiens | |
0.0721 | - |
C20:1-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.0773 | - |
C18:1-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.0814 | - |
D-erythro-C24:1-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.0915 | - |
C20:0-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.0948 | - |
C18:0-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.0985 | - |
C16:0-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.1146 | - |
C14:0-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.1338 | - |
C12:0-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.1432 | - |
C24:0-ceramide | pH 9.0, 37°C | Homo sapiens | |
0.2242 | - |
C6:0-ceramide | pH 9.0, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | ACER2 has 7 putative transmembrane domains, ACER2 membrane topology, overview. The N-terminal tail is necessary for both ACER2 activity and Golgi localization | Homo sapiens | 139 | - |
microsome | - |
Homo sapiens | - |
- |
additional information | ACER2 C- and N-termini are oriented in the lumen of the Golgi complex and cytosol, respectively | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, required by ACER2 for both its in vitro and cellular activities. Cellular ACER2 activity is inhibited by depletion of Ca2+ from Golgi lumen | Homo sapiens | |
additional information | Mg2+ and Mn2+ have no effect on ceramidase activity in ACER2 microsomes | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
C12:0-ceramide + H2O | Homo sapiens | - |
laurate + sphingosine | - |
? | |
C14:0-ceramide + H2O | Homo sapiens | - |
myristate + sphingosine | - |
? | |
C16:0-ceramide + H2O | Homo sapiens | - |
palmitate + sphingosine | - |
? | |
C18:0-ceramide + H2O | Homo sapiens | - |
stearate + sphingosine | - |
? | |
C18:1-ceramide + H2O | Homo sapiens | - |
oleate + sphingosine | - |
? | |
C20:0-ceramide + H2O | Homo sapiens | - |
eicosanoic acid + sphingosine | - |
? | |
C20:1-ceramide + H2O | Homo sapiens | - |
C20:1 fatty acid + sphingosine | - |
? | |
C24:0-ceramide + H2O | Homo sapiens | - |
C24:0 fatty acid + sphingosine | - |
? | |
C6:0-ceramide + H2O | Homo sapiens | - |
hexanoate + sphingosine | - |
? | |
D-erythro-C24:1-ceramide + H2O | Homo sapiens | - |
C24:1 fatty acid + D-erythro-sphingosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C12:0-ceramide + H2O | - |
Homo sapiens | laurate + sphingosine | - |
? | |
C14:0-ceramide + H2O | - |
Homo sapiens | myristate + sphingosine | - |
? | |
C16:0-ceramide + H2O | - |
Homo sapiens | palmitate + sphingosine | - |
? | |
C18:0-ceramide + H2O | - |
Homo sapiens | stearate + sphingosine | - |
? | |
C18:1-ceramide + H2O | - |
Homo sapiens | oleate + sphingosine | - |
? | |
C20:0-ceramide + H2O | - |
Homo sapiens | eicosanoic acid + sphingosine | - |
? | |
C20:1-ceramide + H2O | - |
Homo sapiens | C20:1 fatty acid + sphingosine | - |
? | |
C24:0-ceramide + H2O | - |
Homo sapiens | C24:0 fatty acid + sphingosine | - |
? | |
C24:1-ceramide + H2O | - |
Homo sapiens | C24:1 fatty acid + sphingosine | - |
? | |
C6:0-ceramide + H2O | - |
Homo sapiens | hexanoate + sphingosine | - |
? | |
D-erythro-C24:1-ceramide + H2O | - |
Homo sapiens | C24:1 fatty acid + D-erythro-sphingosine | - |
? | |
additional information | ACER2 shows a broader substrate specificity catalyzing the hydrolysis of various ceramides. The luminal ACER2 N-terminal tail, comprising 36 amino acid residues, is important for ACER2 activity, but the terminal 13 amino acid residues are not required for its activity | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the luminal ACER2 N-terminal tail, comprising 36 amino acid residues, is important for ACER2 activity, but the terminal 13 amino acid residues are not required for its activity | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ACER2 | - |
Homo sapiens |
alkaline ceramidase 2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | ACER2 plays an important role in cellular responses by regulating the hydrolysis of ceramides in cells, activity regulation, overview | Homo sapiens |