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Literature summary for 3.5.1.23 extracted from

  • Xu, R.; Sun, W.; Jin, J.; Obeid, L.M.; Mao, C.
    Role of alkaline ceramidases in the generation of sphingosine and its phosphate in erythrocytes (2010), FASEB J., 24, 2507-2515.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.23

Protein Variants

Protein Variants Comment Organism
additional information knockdown of ACER1, ACER2, and ACEr3 in K562 cells by siRNA and lentiviral shRNA transduction. Transduction with lentiviral particles expressing an ACER3-specific shRNA decreases ACER3 mRNA but increased ACER2 mRNA in K562 cells, and transduction with lentiviral particles expressing ACER2-specific shRNA decreases ACER2 mRNA but increased ACER1 mRNA and vice versa. Thus knocking down the expression of one alkaline ceramidase up-regulates another Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(1S,2R)-D-erythro-2-(N-myristoylamino)-1-phenyl-1-propanol i.e. D-e-MAPP, an alkaline ceramidase inhibitor, that potently and specifically inhibits ACER1, ACER2, and ACER3 activity Homo sapiens
(1S,2R)-D-erythro-2-(N-myristoylamino)-1-phenyl-1-propanol i.e. D-e-MAPP, an alkaline ceramidase inhibitor, that potently and specifically inhibits ACER activity Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-oleoyl-D-erythro-ceramide + H2O Mus musculus common substrate in erythrocytes oleate + D-erythro-sphingosine
-
 ?
N-oleoyl-D-erythro-ceramide + H2O Homo sapiens common substrate in erythrocytes oleate + D-erythro-sphingosine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 five isozymes: acid ceramidase, neutral ceramidase, and three alkaline ceramidases ACER1, ACER2, and ACER3
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Mus musculus
-
erythrocyte express only alkaline ceramidases Homo sapiens
-
K-562 cell alkaline ceramidase activity and the generation of sphingosine and sphingosine-1-phosphate are increased during erythroid differentiation in K562 erythroleukemic cells, e.g. induced by chemotherapeutic agent cytosine arabinoside, Ara-C Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-oleoyl-D-erythro-ceramide + H2O common substrate in erythrocytes Mus musculus oleate + D-erythro-sphingosine
-
 ?
N-oleoyl-D-erythro-ceramide + H2O common substrate in erythrocytes Homo sapiens oleate + D-erythro-sphingosine
-
 ?
N-oleoyl-D-erythro-ceramide + H2O i.e. D-e-C18:1-ceramide, common substrate in erythrocytes Mus musculus oleate + D-erythro-sphingosine
-
 ?
N-oleoyl-D-erythro-ceramide + H2O i.e. D-e-C18:1-ceramide, common substrate in erythrocytes Homo sapiens oleate + D-erythro-sphingosine
-
 ?
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-phytosphingosine + H2O i.e. D-ribo-C12-NBD-phytoceramide Homo sapiens N-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]laurate + phytosphingosine
-
 ?

Synonyms

Synonyms Comment Organism
ACER
-
 Mus musculus
ACER1
-
 Homo sapiens
ACER2
-
 Homo sapiens
ACER3
-
 Homo sapiens
acid ceramidase
-
 Homo sapiens
alkaline ceramidase
-
 Mus musculus
alkaline ceramidase
-
 Homo sapiens
neutral ceramidase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 acid ceramidase, assay at Homo sapiens
7
-
 neutral ceramidase, assay at Homo sapiens
9.4
-
 alkaline ceramidase, assay at Homo sapiens

General Information

General Information Comment Organism
additional information alkaline ceramidase overexpression along with sphingosine 1-phosphate lyase knockdown results in sphingosine 1-phosphate accumulation in cells Homo sapiens
physiological function alkaline ceramidases have a compensatory role in controlling sphingosine and sphingosine 1-phosphate generation in erythroid cells Mus musculus
physiological function alkaline ceramidases have a compensatory role in controlling sphingosine and sphingosine 1-phosphate generation in erythroid cells Homo sapiens