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Literature summary for 3.5.1.23 extracted from
- Molecular cloning and characterization of OsCDase, a ceramidase enzyme from rice (2008), Plant J., 55, 1000-1009.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the yeast expression vector pYES2/CT | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03934 | - |
D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide | apparent KM | Oryza sativa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Oryza sativa | 5783 | - |
| Golgi apparatus | - |
Oryza sativa | 5794 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enhances activity | Oryza sativa | |
| Fe2+ | inhibits activity | Oryza sativa | |
| Mg2+ | enhances activity | Oryza sativa | |
| Mn2+ | enhances activity | Oryza sativa | |
| Zn2+ | enhances activity | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
determined by SDS-PAGE and Western blot analysis, signal between 75 and 100 kDa | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ceramide + H2O | Oryza sativa | - |
sphingosine + fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ceramide + H2O | - |
Oryza sativa | sphingosine + fatty acid | - |
? | |
| D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O | substrate activity assay | Oryza sativa | D-erythro-sphingosine + ? | - |
? | |
| D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-phytoceramide + H2O | substrate activity assay | Oryza sativa | D-erythro-sphingosine + ? | - |
? | |
| palmitoyl sphingosine + H2O | substrate activity assay | Oryza sativa | palmitate + sphingosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ceramidase | - |
Oryza sativa |
| OsCDase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 6 | - |
Oryza sativa |
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Release 2023.1 (January 2023)
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