Cloned (Comment) | Organism |
---|---|
gene Asah2, DNA and amino acid sequence determination and analysis, expression in ES cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of disruption mutant of Asah2 by using a targeted ES clone to produce chimeras and subsequently heterozygous mice, interbreeding of the Asah2 heterozygous mice yielded wild-type, heterozygous, and homozygous mutant null offspring, Asah2 null mice are deficient in the intestinal degradation of ceramide, Asah2 null mice have a normal life span and do not show obvious abnormalities or major alterations in total ceramide levels in tissues | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border | - |
Mus musculus | 5903 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acyl-sphingosine + H2O | Mus musculus | the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract | a carboxylate + sphingosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
gene Asah2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
cecum | - |
Mus musculus | - |
colon | - |
Mus musculus | - |
duodenum | - |
Mus musculus | - |
ileum | - |
Mus musculus | - |
jejunum | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
additional information | tissue expression pattern, oveview | Mus musculus | - |
small intestine | high enzyme expresion level along the brush border | Mus musculus | - |
stomach | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of sphingolipid levels by mass spectrometry | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O | - |
Mus musculus | 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide | - |
? | |
N-acyl-sphingosine + H2O | - |
Mus musculus | a carboxylate + sphingosine | - |
? | |
N-acyl-sphingosine + H2O | the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract | Mus musculus | a carboxylate + sphingosine | - |
? | |
palmitoyl-D-[erythro-9,10]sphingosine + H2O | - |
Mus musculus | palmitate + D-erythro-sphingosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral ceramidase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |