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Literature summary for 3.5.1.23 extracted from
- Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids (2006), J. Biol. Chem., 281, 7324-7331.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Asah2, DNA and amino acid sequence determination and analysis, expression in ES cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of disruption mutant of Asah2 by using a targeted ES clone to produce chimeras and subsequently heterozygous mice, interbreeding of the Asah2 heterozygous mice yielded wild-type, heterozygous, and homozygous mutant null offspring, Asah2 null mice are deficient in the intestinal degradation of ceramide, Asah2 null mice have a normal life span and do not show obvious abnormalities or major alterations in total ceramide levels in tissues | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| brush border | - |
Mus musculus | 5903 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acyl-sphingosine + H2O | Mus musculus | the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract | a carboxylate + sphingosine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
gene Asah2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| cecum | - |
Mus musculus | - |
| colon | - |
Mus musculus | - |
| duodenum | - |
Mus musculus | - |
| ileum | - |
Mus musculus | - |
| jejunum | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| liver | - |
Mus musculus | - |
| additional information | tissue expression pattern, oveview | Mus musculus | - |
| small intestine | high enzyme expresion level along the brush border | Mus musculus | - |
| stomach | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
determination of sphingolipid levels by mass spectrometry | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O | - |
Mus musculus | 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide | - |
? | |
| N-acyl-sphingosine + H2O | - |
Mus musculus | a carboxylate + sphingosine | - |
? | |
| N-acyl-sphingosine + H2O | the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract | Mus musculus | a carboxylate + sphingosine | - |
? | |
| palmitoyl-D-[erythro-9,10]sphingosine + H2O | - |
Mus musculus | palmitate + D-erythro-sphingosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral ceramidase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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