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Literature summary for 3.5.1.23 extracted from
- Rat intestinal ceramidase: purification, properties, and physiological relevance (2004), Am. J. Physiol. Gastrointest. Liver Physiol., 287, G929-G937.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | a bile salt analog | Rattus norvegicus |  |
| glycocholic acid | - |
Rattus norvegicus | |
| additional information | the enzyme is stimulated by bile salts | Rattus norvegicus | |
| taurocholic acid | - |
Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | over 80% inhibition at 0.2 mM, reversible by EDTA | Rattus norvegicus | |
| additional information | the enzyme is stable to trypsin and chymotrypsin exposure | Rattus norvegicus | |
| Zn2+ | over 90% inhibition at 0.2 mM, reversible by EDTA | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
palmitoyl-sphingosine | pH 8.0, 37°C | Rattus norvegicus | |
| 0.0714 | - |
octanoyl-sphingosine | pH 8.0, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| brush border | - |
Rattus norvegicus | 5903 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not affected by Ca2+, Mg2+, or Mn2+ | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
deglycosylated enzyme, gel filtration | Rattus norvegicus |
| 116000 | - |
glycosylated enzyme, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | the enzyme is important in digestion of sphingolipids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native intestinal enzyme over 307fold by acetone precipitation and ion-exchange, hydrophobic-interaction chromatography, and gel filtration, to homogeneity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | lumen | Rattus norvegicus | - |
| small intestine | mucosa, the enzyme is exposed at the brush border | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is important in digestion of sphingolipids | Rattus norvegicus | ? | - |
? | |
| octanoyl-sphingosine + H2O | - |
Rattus norvegicus | octanoate + sphingosine | - |
? | |
| palmitoyl-sphingosine + H2O | - |
Rattus norvegicus | palmitate + sphingosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral ceramidase 2 | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | broad optimum | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | activity range | Rattus norvegicus |
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Release 2023.1 (January 2023)
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