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Literature summary for 3.5.1.23 extracted from

  • Nieuwenhuizen, W.F.; van Leeuwen, S.; Gotz, F.; Egmond, M.R.
    Synthesis of a novel fluorescent ceramide analogue and its use in the characterization of recombinant ceramidase from Pseudomonas aeruginosa PA01 (2002), Chem. Phys. Lipids, 114, 181-191.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
sphingosine and sphingosine analogues Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
(4E,2S,3R)-2-N-(10-pyrenedecanoyl)-1,3,17-trihydroxy-17-(3,5-dinitrobenzoyl)-4-heptadecene
-
Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information divalent cation required, without strong specificity Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acylsphingosine + H2O Pseudomonas aeruginosa involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate a carboxylate + sphingosine
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
strain PA01
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4E,2S,3R)-2-N-(10-pyrenedecanoyl)-1,3,17-trihydroxy-17-(3,5-dinitrobenzoyl)-4-heptadecene + H2O
-
Pseudomonas aeruginosa ? fluorescent product formed upon hydrolysis of the N-acyl bond ?
N-acylsphingosine + H2O
-
Pseudomonas aeruginosa a carboxylate + sphingosine
-
?
N-acylsphingosine + H2O involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate Pseudomonas aeruginosa a carboxylate + sphingosine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
(4E,2S,3R)-2-N-(10-pyrenedecanoyl)-1,3,17-trihydroxy-17-(3,5-dinitrobenzoyl)-4-heptadecene
-
Pseudomonas aeruginosa