Any feedback?
Literature summary for 3.5.1.23 extracted from
- Structural requirements of ceramide and sphingosine based inhibitors of mitochondrial ceramidase (2001), Biochemistry, 40, 9657-9668.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ceramide-1-phosphate | stimulates | Rattus norvegicus |  |
| sphingosine-1-phosphate | stimulates | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (2S)-3-keto-dehydrosphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
| (2S)-3-keto-hexadecanoyl-ceramide | mtCDase, IC50: 0.6 mol% | Rattus norvegicus | |
| (2S)-3-keto-sphinganine | mtCDase, IC50: 0.34 mol% | Rattus norvegicus | |
| 1-O-methyl-D-erythro-sphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
| C18-ceramide | mtCDase, competitive inhibition, IC50: 0.62 mol% | Rattus norvegicus | |
| cis-D-erythro-sphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
| D-erythro-dehydrosphingosine | mtCDase, IC50: 0.25 mol% | Rattus norvegicus | |
| D-erythro-sphinganine | mtCDase, IC50: 0.2 mol% | Rattus norvegicus | |
| D-erythro-sphingosine | IC50: 0.04 mol%, mt-CDase is inhibited by all stereoisomers of sphingosine with IC50 ranging from 0.04 to 0.14 mol% | Rattus norvegicus | |
| D-erythro-urea-C16-ceramide | mtCDase, competitive inhibition, IC50: 0.33 mol% | Rattus norvegicus | |
| D-threo-ceramide | mt-CDase, IC50: 0.21 mol% | Rattus norvegicus | |
| L-erythro-ceramide | mt-CDase, IC50: 0.26 mol% | Rattus norvegicus | |
| L-threo-ceramide | mt-CDase, IC50: 0.11 mol% | Rattus norvegicus | |
| additional information | not inhibited by N-methyl-hexadecanoyl-ceramide, 1-O-methyl-hexadecanoyl-ceramide, 3-O-methyl-hexadecanoyl-ceramide, cis-D-erythro-hexadecanoyl-ceramide, 3-O-methyl-D-erythro-sphingosine, requirements for inhibition of mtCDase | Rattus norvegicus | |
| N,N-dimethyl-D-erythro-sphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
| N-methyl-D-erythro-sphingosine | mtCDase, IC50: 0.13 mol% | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-octadecanoyl-ceramide + H2O | mtCDase specifically hydrolyzes the D-erythro-isomer of ceramide, requirements for ceramide-enzyme interaction, ligand interaction with the enzyme occurs in a high affinity low specificity manner, in contrast to catalysis which is highly specific for D-erythro-ceramide but occurs with a lower affinity | Rattus norvegicus | D-erythro-sphingosine + palmitic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mt-CDase | mitochondrial ceramidase | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
assay at | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
