Any feedback?
Literature summary for 3.5.1.23 extracted from
- Purification and characterization of a novel ceramidase from Pseudomonas aeruginoa (1998), J. Biol. Chem., 273, 14368-14373.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas aeruginosa |  |
| Triton X-100 | - |
Pseudomonas aeruginosa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Pseudomonas aeruginosa | |
| EDTA | - |
Pseudomonas aeruginosa | |
| EGTA | - |
Pseudomonas aeruginosa | |
| Hg2+ | - |
Pseudomonas aeruginosa | |
| N-oleoylethanolamine | - |
Pseudomonas aeruginosa | |
| phytosphingosine | - |
Pseudomonas aeruginosa | |
| sphinganine | - |
Pseudomonas aeruginosa | |
| sphingosine | - |
Pseudomonas aeruginosa | |
| Zn2+ | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.139 | - |
N-palmitoylsphingosine | - |
Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | after EDTA treatment, Ca2+ reestablishes activity completely | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 83400fold | Pseudomonas aeruginosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Pseudomonas aeruginosa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 186 | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, 2 months, with 1 mg/ml bovine serum albumin, no loss of activity | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-nitrobenz-2-oxa-1,3-diazole-N-lauroylsphingosine + H2O | - |
Pseudomonas aeruginosa | ? | - |
r | |
| N-palmitoylphytosphingosine + H2O | - |
Pseudomonas aeruginosa | ? | - |
r | |
| N-palmitoylsphinganine + H2O | - |
Pseudomonas aeruginosa | palmitic acid + sphinganine | - |
r | |
| N-palmitoylsphingosine + H2O | - |
Pseudomonas aeruginosa | palmitic acid + sphingosine | - |
r | |
| N-stearoylphytosphingosine + H2O | - |
Pseudomonas aeruginosa | ? | - |
r | |
| N-stearoylsphinganine + H2O | - |
Pseudomonas aeruginosa | stearic acid + sphinganine | - |
r | |
| N-stearoylsphingosine + H2O | - |
Pseudomonas aeruginosa | stearic acid + sphingosine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
stable at 24°C for 12 h | Pseudomonas aeruginosa |
| 37 | - |
total loss of activity after 12 h at 37°C | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
