Application | Comment | Organism |
---|---|---|
food industry | microbial glutaminases are enzymes with emerging potential in both the food and the pharmaceutical industries, potential application for bioconversion of glutamine to flavor-enhancing glutamic acid | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
gene glsA, expression of His10-tagged GlsA in Escherichia coli under the control of a ptac promoter | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration | Bacillus licheniformis |
38500 | - |
1 * 38500, about, sequence calculation, 1 * 39000, SDS-PAGE | Bacillus licheniformis |
39000 | - |
1 * 38500, about, sequence calculation, 1 * 39000, SDS-PAGE | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Bacillus licheniformis | - |
L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Bacillus licheniformis DSM 13 | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q65NY1 | ATCC 14580 | - |
Bacillus licheniformis DSM 13 | Q65NY1 | ATCC 14580 | - |
Purification (Comment) | Organism |
---|---|
recombinant His10-tagged GlsA 2.7fold from Escherichia coli by nickel affinity chromatography to homogeneity | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
325 | - |
purified recombinant His10-tagged GlsA, pH 7.5, 30°C | Bacillus licheniformis |
Storage Stability | Organism |
---|---|
30°C, pH 7.5, purified recombinant enzyme, stable for up to 6 h | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Bacillus licheniformis | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Bacillus licheniformis DSM 13 | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38500, about, sequence calculation, 1 * 39000, SDS-PAGE | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
GlsA | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
physiological function | glutaminase functions in cellular metabolism of every organism by supplying nitrogen required for the biosynthesis of a variety of metabolic intermediates, while glutamic acid plays a role in both sensory and nutritional properties of food | Bacillus licheniformis |