Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, at 25% NaCl concentration, the enzyme retains 60% of its activity after 4 h | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-glutamine | pH 11.0, 70°C | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, optimal at 1% | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Bacillus sp. (in: Bacteria) | - |
L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Bacillus sp. (in: Bacteria) LKG-01 | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
MTCC 10401, isolated from Gangotri region of Uttarakhand Himalaya | - |
Bacillus sp. (in: Bacteria) LKG-01 | - |
MTCC 10401, isolated from Gangotri region of Uttarakhand Himalaya | - |
Purification (Comment) | Organism |
---|---|
native enzyme, 49fold from cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
584.2 | - |
purified enzyme, pH 11.0, 70°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glutamine + H2O | low activity | Bacillus sp. (in: Bacteria) | D-glutamate + NH3 | - |
? | |
D-glutamine + H2O | low activity | Bacillus sp. (in: Bacteria) LKG-01 | D-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Bacillus sp. (in: Bacteria) | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | preferred substrate | Bacillus sp. (in: Bacteria) | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Bacillus sp. (in: Bacteria) LKG-01 | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | preferred substrate | Bacillus sp. (in: Bacteria) LKG-01 | L-glutamate + NH3 | - |
? | |
L-glutaminyl-L-asparagine + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
L-glutaminyl-L-asparagine + H2O | - |
Bacillus sp. (in: Bacteria) LKG-01 | ? | - |
? | |
additional information | no activity with D-asparagine | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with D-asparagine | Bacillus sp. (in: Bacteria) LKG-01 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
L-glutaminase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 85 | activity range | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, 3 h, 100% stable | Bacillus sp. (in: Bacteria) |
70 | - |
purified enzyme, 3 h, 50% activity remaining | Bacillus sp. (in: Bacteria) |
80 | - |
purified enzyme, 3 h, 20% activity remaining | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 12 | activity range | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | purified enzyme, stable | Bacillus sp. (in: Bacteria) |