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Literature summary for 3.5.1.2 extracted from
- Partial purification and characterization of glutaminase from Lactobacillus reuteri KCTC3594 (2010), Appl. Biochem. Biotechnol., 162, 146-154.
General Stability
| General Stability | Organism |
|---|---|
| enzyme shows high instability especially during the gel filtration step. Addition of 10% ethylene glycol as stabilizer | Limosilactobacillus reuteri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-diazo-5-oxo-L-norleucine | 10 mM, strongly inhibited, 80% inhibition, especially more strongly with a progress of purification steps | Limosilactobacillus reuteri |  |
| iodoacetate | 50 mM, strongly inhibited, 80% inhibition, especially more strongly with a progress of purification steps | Limosilactobacillus reuteri | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | bound to membrane, highest activity in the cell pellet in comparison to supernatant fraction | Limosilactobacillus reuteri | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | salt-tolerant, 110% relative activity under 5% NaCl, 50% activity at 15% (w/v) concentration, over 50% activity with presence of 20% NaCl | Limosilactobacillus reuteri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
and 70000 Da, SDS-PAGE | Limosilactobacillus reuteri |
| 70000 | - |
and 50000 Da, SDS-PAGE | Limosilactobacillus reuteri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus reuteri | - |
KCTC3594 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified, 21fold, 0.2% yield, protamine sulfate treatment, anion exchange chromatography, and gel filtration | Limosilactobacillus reuteri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.4 | - |
pH 7.5, 37°C | Limosilactobacillus reuteri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Limosilactobacillus reuteri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | over 70% activity at 20°C and ca. 90% at 60°C | Limosilactobacillus reuteri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
78% activity remains after incubation for 10 min at 60°C | Limosilactobacillus reuteri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Limosilactobacillus reuteri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | over 40% activity at pH 5 and over 70"% at pH 11 | Limosilactobacillus reuteri |
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Release 2023.1 (January 2023)
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