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Literature summary for 3.5.1.2 extracted from
- Characterization of two glutaminases from the filamentous cyanobacterium Anabaena sp. PCC 7120 (2008), FEMS Microbiol. Lett., 289, 241-249.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Anabaena sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.5 | - |
glutamine | Km apparent, no Michaelis-Menten kinetics | Anabaena sp. |  |
| 109.5 | - |
glutamine | Km apparent, no Michaelis-Menten kinetics | Anabaena sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | no activity without phosphate, max. activity at 60 mM K3PO4 | Anabaena sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Anabaena sp. |
| 36000 | - |
x * 36000, SDS-PAGE | Anabaena sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena sp. | Q8YMZ9 | strain PCC 7120 | - |
| Anabaena sp. | Q8YSZ5 | strain PCC 7120 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Anabaena sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.71 | - |
Vmax apparent, no Michaelis-Menten kinetics | Anabaena sp. |
| 102.3 | - |
Vmax apparent, no Michaelis-Menten kinetics | Anabaena sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | - |
Anabaena sp. | L-glutamic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Anabaena sp. |
| ? | x * 33000, SDS-PAGE | Anabaena sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutaminase | - |
Anabaena sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Anabaena sp. |
| 37 | - |
- |
Anabaena sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Anabaena sp. |
| 7.5 | - |
- |
Anabaena sp. |
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