Cloned (Comment) | Organism |
---|---|
the L gene, encoding the liver-type isozyme, is cloned from a brain library and is located on chromosome 12, while the kidney-type isozyHomo sapiens |
|
Protein Variants | Comment | Organism |
---|---|---|
E599A | site-directed mutagenesis | Homo sapiens |
M601A | site-directed mutagenesis | Homo sapiens |
S600A | site-directed mutagenesis | Homo sapiens |
V602A | site-directed mutagenesis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-glutamate | strong product inhibition of isozyme KGA, no inhibition of isozyme LGA | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | of neutrophils, isozyme LGA | Homo sapiens | 9986 | - |
extracellular | isozyme LGA is secreted from the plasma membrane/cell surface of neutrophils | Homo sapiens | - |
- |
mitochondrion | isozyme LGA | Homo sapiens | 5739 | - |
mitochondrion | isozyme LGA | Rattus norvegicus | 5739 | - |
additional information | analysis of subcellular targeting sequences and motifs of isozyme LGA | Homo sapiens | - |
- |
nucleus | isozyme LGA, in brain | Homo sapiens | 5634 | - |
nucleus | isozyme LGA, in brain | Rattus norvegicus | 5634 | - |
plasma membrane | of neutrophils, isozyme LGA | Homo sapiens | 5886 | - |
secretory granule | isozyme LGA | Homo sapiens | 30141 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | phosphate-activated glutaminase isozyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Rattus norvegicus | the enzyme plays a key role in energy and nitrogen metabolism | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Homo sapiens | the enzyme plays a key role in energy and nitrogen metabolism, glutamate is a major excitatory neurotransmitter in neurons, especially in the cerebral cortex | L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
two isozymes, glutaminase K and L, i.e. LGA and KGA, or liver-type isozyme and kidney-type isozyme | - |
no activity in Gallus gallus | - |
no liver-type isozyme, LGA, activity in the brain | - |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | isozymes LGA and KGA | Homo sapiens | - |
brain | isozymes LGA and KGA | Rattus norvegicus | - |
brain | isozymes LGA and KGA, mainly astrocytes, cerebral cortex, and neurons | Homo sapiens | - |
breast cancer cell | - |
Homo sapiens | - |
cerebral cortex | isozymes LGA and KGA | Homo sapiens | - |
colonic cancer cell | splicing variant isozyme GAC | Homo sapiens | - |
kidney | isozyme KGA | Homo sapiens | - |
kidney | isozyme KGA and splicing variant isozyme GAC | Rattus norvegicus | - |
liver | isozyme LGA is specific for liver, and isozyme KGA is expressed in fetal liver | Rattus norvegicus | - |
liver | isozymes LGA and KGA | Homo sapiens | - |
lymphocyte | isozyme KGA | Rattus norvegicus | - |
additional information | isozyme expression patterns | Rattus norvegicus | - |
additional information | isozyme tissue-specific expression patterns, the enzyme is overexpressed in a variety of tumours and malignancies, overview | Homo sapiens | - |
neuron | isozymes LGA and KGA | Homo sapiens | - |
neutrophil | isozyme LGA is secreted from the plasma membrane | Homo sapiens | - |
pancreas | isozymes LGA and KGA | Rattus norvegicus | - |
small intestine | isozyme KGA | Rattus norvegicus | - |
small intestine | isozymes LGA and KGA | Homo sapiens | - |
spinal cord | low expression level of isozymes LGA and KGA | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Homo sapiens | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Rattus norvegicus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | the enzyme plays a key role in energy and nitrogen metabolism | Rattus norvegicus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | the enzyme plays a key role in energy and nitrogen metabolism, glutamate is a major excitatory neurotransmitter in neurons, especially in the cerebral cortex | Homo sapiens | L-glutamate + NH3 | - |
? | |
additional information | multifunctional isozyme LGA interacts with PDZ domain proteins, e.g. alpha-1-syntrophin or glutaminase-interacting protein GIP, via specific short sequence motifs, interaction mechanism, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Homo sapiens |
GA | - |
Rattus norvegicus |
GAC | - |
Homo sapiens |
GAC | - |
Rattus norvegicus |
glutaminase K | - |
Homo sapiens |
glutaminase K | - |
Rattus norvegicus |
glutaminase L | - |
Homo sapiens |
glutaminase L | - |
Rattus norvegicus |
KAG | - |
Homo sapiens |
KAG | - |
Rattus norvegicus |
LAG | - |
Homo sapiens |
LAG | - |
Rattus norvegicus |
phosphate-activated glutaminase | - |
Homo sapiens |
phosphate-activated glutaminase | - |
Rattus norvegicus |