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Literature summary for 3.5.1.2 extracted from

  • Soda, K.; Oshima, M.; Yamamoto, T.
    Purification and properties of isozymes of glutaminase from Pseudomonas aeruginosa (1972), Biochem. Biophys. Res. Commun., 46, 1278-1284.
    View publication on PubMed
Search for more literature for 3.5.1.2

Crystallization (Commentary)

Crystallization (Comment) Organism
by addition of ammonium sulfate Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
 Pseudomonas aeruginosa
additional information no inhibition by HgCl2; no inhibition by p-chloromercuribenzoate Pseudomonas aeruginosa
p-chloromercuribenzoate
-
 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 L-glutamine
-
 Pseudomonas aeruginosa
0.18
-
 L-glutamine
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 gel filtration Pseudomonas aeruginosa
137000
-
 sedimentation equilibrium mehod Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Pseudomonas aeruginosa
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 IFO 3080
-
Pseudomonas aeruginosa Q9I387 IFO 3080
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.1
-
 purified enzyme Pseudomonas aeruginosa
27.1
-
 purified enzyme Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little lose of activity Pseudomonas aeruginosa
0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-asparagine + H2O 44% activity Pseudomonas aeruginosa D-aspartate + NH3
-
 ?
D-glutamine + H2O 65% activity Pseudomonas aeruginosa D-glutamate + NH3
-
 ?
D-glutamine + H2O 92% activity Pseudomonas aeruginosa D-glutamate + NH3
-
 ?
D-theanine + H2O 57% activity Pseudomonas aeruginosa D-glutamate + ethylamine
-
 ?
L-asparagine + H2O 51% activity Pseudomonas aeruginosa L-aspartate + NH3
-
 ?
L-gamma-glutamyl-p-nitroanilide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
L-gamma-glutamylhydrazine + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
L-glutamine + H2O
-
 Pseudomonas aeruginosa L-glutamate + NH3
-
 ?
L-glutamine + H2O pH 7.2, 30°C Pseudomonas aeruginosa L-glutamate + NH3
-
 ?
L-theanine + H2O 71% activity Pseudomonas aeruginosa L-glutamate + ethylamine
-
 ?
additional information no reaction with L-theanine, D-theanine, L-gamma-glutamylhydrazine, and L-gamma-glutamyl-p-nitroanilide Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
glutaminase A
-
 Pseudomonas aeruginosa
glutaminase B
-
 Pseudomonas aeruginosa
L-glutamine amidohydrolase
-
 Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9
-
 Pseudomonas aeruginosa