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Literature summary for 3.5.1.2 extracted from

  • Nandakumar, R.; Wakayama, M.; Nagano, Y.; Kawamura, T.; Sakai, K.; Moriguchi, M.
    Overexpression of salt-tolerant glutaminase from Micrococcus luteus K-3 in Escherichia coli and its purification (1999), Protein Expr. Purif., 15, 155-161.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Micrococcus luteus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Micrococcus luteus

General Stability

General Stability Organism
stabilized by MgCl2 and glycerol in addition to NaCl Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Micrococcus luteus
NaCIO4 0.3 M, 25% inhibition Micrococcus luteus
NaH2PO4 0.3 M, complete inhibition Micrococcus luteus
NaI above 0.3 M Micrococcus luteus
NaNO3 0.3 M, 15% inhibition Micrococcus luteus
PCMB
-
Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 2 M, 1.4fold activation Micrococcus luteus
NaBr 0.25 M, 1.2-1.3fold activation Micrococcus luteus
NaF 0.25 M, 1.2-1.3fold activation Micrococcus luteus
NaI 0.25 M, 1.2-1.3fold activation Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Gln + H2O Micrococcus luteus
-
L-Glu + NH3
-
?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.23
-
recombinant enzyme Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gln + H2O
-
Micrococcus luteus Glu + NH3
-
?
L-Gln + H2O
-
Micrococcus luteus L-Glu + NH3
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Micrococcus luteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Micrococcus luteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
in presence of 1.71 M NaCl, stable up to Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Micrococcus luteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9 stable Micrococcus luteus