Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Micrococcus luteus |
Crystallization (Comment) | Organism |
---|---|
- |
Micrococcus luteus |
General Stability | Organism |
---|---|
stabilized by MgCl2 and glycerol in addition to NaCl | Micrococcus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Micrococcus luteus | |
NaCIO4 | 0.3 M, 25% inhibition | Micrococcus luteus | |
NaH2PO4 | 0.3 M, complete inhibition | Micrococcus luteus | |
NaI | above 0.3 M | Micrococcus luteus | |
NaNO3 | 0.3 M, 15% inhibition | Micrococcus luteus | |
PCMB | - |
Micrococcus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 2 M, 1.4fold activation | Micrococcus luteus | |
NaBr | 0.25 M, 1.2-1.3fold activation | Micrococcus luteus | |
NaF | 0.25 M, 1.2-1.3fold activation | Micrococcus luteus | |
NaI | 0.25 M, 1.2-1.3fold activation | Micrococcus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Gln + H2O | Micrococcus luteus | - |
L-Glu + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Micrococcus luteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.23 | - |
recombinant enzyme | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gln + H2O | - |
Micrococcus luteus | Glu + NH3 | - |
? | |
L-Gln + H2O | - |
Micrococcus luteus | L-Glu + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Micrococcus luteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Micrococcus luteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in presence of 1.71 M NaCl, stable up to | Micrococcus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Micrococcus luteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | stable | Micrococcus luteus |