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Literature summary for 3.5.1.19 extracted from

  • Ion, B.F.; Kazim, E.; Gauld, J.W.
    A multi-scale computational study on the mechanism of Streptococcus pneumoniae nicotinamidase (SpNic) (2014), Molecules, 19, 15735-15753.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.19

Application

Application Comment Organism
drug development while nicotinamidases are widespread in nature they are not occuring within mammals and present a potential drug target. The enzymes can be used in the activation of tuberculosis prodrugs such as pyrazinamide Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-dependent enzyme Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide + H2O Streptococcus pneumoniae
-
 nicotinate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
nicotinamide + H2O = nicotinate + NH3 catalytic mechanism in two stages: (i) formation of a tetrahedral thioester enzyme-substrate intermediate (with the sulfur of Cys136 nucleophilically attacks the nicotinamidase substrate's carbonyl carbon while concomitantly the Cys136-SH thiol proton is transferred onto the substrate's amide group nitrogen) and (ii) hydrolysis of the thioester bond to give the products, overview. The catalytic triad comprises Lys103, Asp9, and Cys136, substrate binding and roles of active site residues of the enzyme from Streptococcus pneumoniae. The active site Lys103 likely plays a role in stabilizing the thiolate of Cys136 during the reaction Streptococcus pneumoniae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O
-
 Streptococcus pneumoniae nicotinate + NH3
-
 ?
pyrazinamide + H2O
-
 Streptococcus pneumoniae pyrazinoic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
NIC
-
 Streptococcus pneumoniae
nicotinamidase
-
 Streptococcus pneumoniae
SpNic
-
 Streptococcus pneumoniae

General Information

General Information Comment Organism
evolution nicotinamidases are a family of peptide hydrolases that generally contain a Zn2+ ion Streptococcus pneumoniae
metabolism nicotinamidase is a key enzyme in NAD+ metabolism Streptococcus pneumoniae
additional information catalytic mechanism, substrate binding and roles of active site residues, quantum mechanics/molecular mechanics methods and modeling, overview. The polar protein environment has a significant effect in stabilizing reaction intermediates and in particular transition states Streptococcus pneumoniae