Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Serum albumin | 0.02%, 9% activation | Mus musculus |
General Stability | Organism |
---|---|
freezing and defreezing inactivates | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Mus musculus | |
Ag+ | - |
Mus musculus | |
Cu2+ | - |
Mus musculus | |
Cys | - |
Mus musculus | |
dithiothreitol | - |
Mus musculus | |
EDTA | 0.02 mM, 46% inhibition | Mus musculus | |
Fe2+ | - |
Mus musculus | |
Hg2+ | - |
Mus musculus | |
NAD+ | competitive with respect to nicotinamide | Mus musculus | |
sulfhydryl reagents | - |
Mus musculus | |
Triton X-100 | 0.1%, 24% inhibition | Mus musculus | |
Urea | 1 M, 20% inhibition | Mus musculus | |
Zn2+ | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mus musculus | |
0.006 | - |
nicotinamide | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Mus musculus | |
Mg2+ | activation | Mus musculus | |
Mn2+ | activation | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 65000 + 2 * 50000, SDS-PAGE | Mus musculus |
65000 | - |
2 * 65000 + 2 * 50000, SDS-PAGE | Mus musculus |
230000 | - |
gel electrophoresis | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | Mus musculus | enzyme is induced at high nicotinamide concentrations | nicotinate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 1.7% w/w mannose and 1.5% w/w N-acetylglucosamine | Mus musculus |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
C-1300 cell | C1300 neuroblastoma M1 clonal cell line | Mus musculus | - |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, stable for months | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | - |
Mus musculus | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | enzyme is induced at high nicotinamide concentrations | Mus musculus | nicotinate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 65000 + 2 * 50000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life: 2.5 min | Mus musculus |
65 | - |
half-life: 1 min | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.5 | - |
Mus musculus |
7.2 | - |
- |
Mus musculus |
7.5 | - |
- |
Mus musculus |