Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression as soluble secreted enzyme in Streptomyces lividans strain TK24, subcloning in Escherichia coli strain DH5alpha | Streptomyces mobaraensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55816 | - |
x * 55816, sequence calculation | Streptomyces mobaraensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | C4B800 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme to homogeneity | Streptomyces mobaraensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2500 | 2800 | purified recombinant enzyme, pH not specified in the publication, temperature not specified in the publication | Streptomyces mobaraensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sm-ELA catalyzes not only the hydrolysis of various Nepsilon-acyl-L-lysines but also the synthesis of Nepsilon-acyl-L-lysines from L-lysine hydrochloride and medium-/long-chain fatty acids, including Nepsilon-lauroyl-L-lysine | Streptomyces mobaraensis | ? | - |
? | |
N6-acyl-L-lysine + H2O | the enzyme specifically catalyzes hydrolysis of the epsilon-amide bond in various Nepsilon-acyl-L-lysines | Streptomyces mobaraensis | a carboxylate + L-lysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55816, sequence calculation | Streptomyces mobaraensis |
Synonyms | Comment | Organism |
---|---|---|
ELA | - |
Streptomyces mobaraensis |
epsilon-lysine acylase | - |
Streptomyces mobaraensis |
More | the enzyme belongs to the YtcJ-like metal-dependent amidohydrolase family, which is further characterized as the metallo-dependent hydrolase superfamily | Streptomyces mobaraensis |