BRENDA - Enzyme Database
show all sequences of 3.5.1.16

Genetic and biochemical characterization of arginine biosynthesis in Sinorhizobium meliloti 1021

Hernandez, V.M.; Girard, L.; Hernandez-Lucas, I.; Vazquez, A.; Ortiz-Ortiz, C.; Diaz, R.; Dunn, M.F.; Microbiology 161, 1671-1682 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli K-12. Because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme
Sinorhizobium meliloti
Inhibitors
Inhibitors
Commentary
Organism
Structure
arginine
-
Sinorhizobium meliloti
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
N2-Acetyl-L-ornithine
pH 8.0, 30°C
Sinorhizobium meliloti
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CoCl2
metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2
Sinorhizobium meliloti
MnCl2
metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2. MnCl2 (0.2 mM) added to the reaction in place of CoCl2 restores activity to about 40 % of that seen with CoCl2
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N2-acetyl-L-ornithine + H2O
Sinorhizobium meliloti
-
acetate + L-ornithine
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Sinorhizobium meliloti
Q92Y75
-
-
Purification (Commentary)
Purification (Commentary)
Organism
because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme
Sinorhizobium meliloti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N2-acetyl-L-ornithine + H2O
-
754657
Sinorhizobium meliloti
acetate + L-ornithine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ArgE
-
Sinorhizobium meliloti
N-acetylornithine deacetylase
-
Sinorhizobium meliloti
NAO deacetylase
-
Sinorhizobium meliloti
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sinorhizobium meliloti
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Sinorhizobium meliloti
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli K-12. Because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme
Sinorhizobium meliloti
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arginine
-
Sinorhizobium meliloti
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
N2-Acetyl-L-ornithine
pH 8.0, 30°C
Sinorhizobium meliloti
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CoCl2
metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2
Sinorhizobium meliloti
MnCl2
metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2. MnCl2 (0.2 mM) added to the reaction in place of CoCl2 restores activity to about 40 % of that seen with CoCl2
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N2-acetyl-L-ornithine + H2O
Sinorhizobium meliloti
-
acetate + L-ornithine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N2-acetyl-L-ornithine + H2O
-
754657
Sinorhizobium meliloti
acetate + L-ornithine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sinorhizobium meliloti
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Sinorhizobium meliloti
General Information
General Information
Commentary
Organism
malfunction
inactivation of argE gene does not cause arginine auxotrophy or marked growth defects
Sinorhizobium meliloti
General Information (protein specific)
General Information
Commentary
Organism
malfunction
inactivation of argE gene does not cause arginine auxotrophy or marked growth defects
Sinorhizobium meliloti
Other publictions for EC 3.5.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755033
Molesini
The Arabidopsis N-acetylornit ...
Arabidopsis thaliana
Plant Cell Physiol.
58
130-144
2017
-
-
-
-
-
-
-
1
-
-
-
1
-
10
-
-
-
-
-
-
-
-
2
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
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1
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1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
1
1
734884
Molesini
Involvement of the putative N- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant Cell Physiol.
56
1084-1096
2015
-
-
1
-
1
-
-
-
-
-
-
2
-
12
-
-
-
-
-
9
-
-
2
-
4
-
-
-
-
-
-
-
-
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-
1
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-
1
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-
-
-
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2
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-
-
9
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
754657
Hernandez
Genetic and biochemical chara ...
Sinorhizobium meliloti
Microbiology
161
1671-1682
2015
-
-
1
-
-
-
1
1
-
2
-
1
-
12
-
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1
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-
-
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1
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3
1
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1
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1
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1
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1
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2
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1
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1
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1
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1
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-
1
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-
-
-
1
1
-
-
-
733082
Hlavacek
Mono-N-acyl-2,6-diaminopimelic ...
Escherichia coli
Anal. Biochem.
467
4-13
2014
-
-
-
-
-
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
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-
4
-
-
-
-
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4
6
-
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1
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-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
735279
McGregor
Identification of a histidine ...
Escherichia coli
SpringerPlus
2
482
2013
-
-
1
-
4
-
-
-
-
2
-
-
-
5
-
-
-
-
1
-
-
-
-
-
2
-
-
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1
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1
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4
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2
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-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
720166
Tao
Structural characterization of ...
Escherichia coli
J. Inorg. Biochem.
111
157-163
2012
-
-
-
-
-
-
-
2
-
-
1
-
-
6
-
-
1
-
-
-
-
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1
1
1
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2
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2
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1
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-
1
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-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
710829
Hlavacek
Inhibitors of N(alpha)-acetyl- ...
Bacillus subtilis, Bacillus subtilis 168
Amino Acids
38
1155-1164
2010
-
-
-
-
-
-
19
-
-
-
-
-
-
98
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
18
19
-
-
-
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-
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-
-
-
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-
-
-
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-
-
-
-
687815
McGregor
Characterization of the cataly ...
Escherichia coli
J. Biol. Inorg. Chem.
12
603-613
2007
-
-
-
-
-
-
1
2
-
1
1
-
-
8
-
-
1
-
-
-
-
-
1
-
2
-
-
1
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
2
-
1
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
669046
McGregor
argE-encoded N-acetyl-L-ornith ...
Escherichia coli, Escherichia coli BL21 (DE3)
J. Am. Chem. Soc.
127
14100-14107
2005
-
1
-
-
-
-
-
2
-
3
3
-
-
15
-
-
1
-
-
-
1
-
2
2
2
-
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
3
3
-
-
-
-
1
-
-
1
-
2
2
-
1
1
2
-
-
-
-
-
-
-
-
-
-
171963
Xu
Evolution of arginine biosynth ...
Escherichia coli, Moritella profunda, Moritella abyssi, Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Bacteriol.
182
1609-1615
2000
-
-
2
-
-
-
-
-
-
-
-
5
-
32
-
-
-
-
-
-
-
-
9
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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5
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-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
171964
Javid-Majd
Mechanistic analysis of the ar ...
Escherichia coli, Escherichia coli 160-37, Escherichia coli ATCC 9637
Biochemistry
39
1285-1293
2000
1
-
1
-
-
-
5
15
-
4
3
1
-
16
-
-
1
-
-
-
-
-
23
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
5
-
15
-
4
3
1
-
-
-
1
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-
-
-
23
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
171961
Boyen
Acetylornithine deacetylase, s ...
Escherichia coli
Gene
116
1-6
1992
-
-
1
-
-
-
-
-
-
2
3
1
-
8
-
-
1
-
-
-
-
-
3
1
1
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-
-
-
-
-
-
-
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-
1
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2
3
1
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1
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3
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
171962
Meinnel
Structural and biochemical cha ...
Escherichia coli, Saccharomyces sp., Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Bacteriol.
174
2323-2331
1992
-
-
1
-
-
-
-
1
-
-
5
4
-
21
-
-
1
-
-
-
1
-
15
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
5
4
-
-
-
1
-
-
1
-
15
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725629
Van de Casteele
-
Pathways of arginine biosynthe ...
Saccharolobus solfataricus, Saccharolobus solfataricus P1
J. Gen. Microbiol.
136
1177-1183
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
2
-
2
-
1
1
-
-
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1
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-
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2
-
2
-
1
-
-
-
1
-
-
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1
1
1
1
-
-
171960
Vogel
Acetylornithinase of Escherich ...
Aerobacter sp., Escherichia coli, Erwinia sp., Klebsiella sp., Proteus sp., Salmonella sp., Serratia sp., Shigella sp., Escherichia coli B / ATCC 11303, Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Biol. Chem.
218
97-106
1956
1
-
-
-
-
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5
-
-
2
-
11
-
199
-
-
1
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-
1
-
33
-
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1
1
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1
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5
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2
-
11
-
-
-
1
-
-
1
-
33
-
-
-
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-
1
1
-
-
-
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