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Literature summary for 3.5.1.16 extracted from
- argE-encoded N-acetyl-L-ornithine deacetylase from Escherichia coli contains a dinuclear metalloactive site (2005), J. Am. Chem. Soc., 127, 14100-14107.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzymatic target for a novel set of antibiotics | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
N2-Acetyl-L-ornithine | incubation with Zn2+ | Escherichia coli |  |
| 1.2 | - |
N2-Acetyl-L-ornithine | incubation with Co2+ | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 34.3 kJ/mol activation energy | Escherichia coli | |
| additional information | 90% of full activity upon addition of one metal ion | Escherichia coli | |
| Zn2+ | 25.6 kJ/mol activation energy | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
4 * 42000, assembles the majority of ArgE | Escherichia coli |
| 42000 | - |
8 * 42000, theoretical calculation | Escherichia coli |
| 170000 | - |
non-denaturing PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli BL21 (DE3) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fast-flow Q-Sepharose column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2000 | - |
2 mM N2-acetyl-L-ornithine at 25 °C and pH 7.5 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N2-acetyl-L-ornithine + H2O | - |
Escherichia coli | L-ornithine + acetate | - |
? | |
| N2-acetyl-L-ornithine + H2O | - |
Escherichia coli BL21 (DE3) | L-ornithine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 42000, theoretical calculation | Escherichia coli |
| tetramer | 4 * 42000, assembles the majority of ArgE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arg-E encoded N-acetyl L-ornithine deacetylase | - |
Escherichia coli |
| ArgE | - |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | 50 mM Chelex 100 treated phosphate buffer at pH 7.5 | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1600 | - |
N2-Acetyl-L-ornithine | in the presence of Zn2+ | Escherichia coli | |
| 3800 | - |
N2-Acetyl-L-ornithine | in the presence of Co2+ | Escherichia coli | |
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Release 2023.1 (January 2023)
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