BRENDA - Enzyme Database
show all sequences of 3.5.1.16

Acetylornithine deacetylase, succinyldiaminopimelate desuccinylase and carboxypeptidase G2 are evolutionarily related

Boyen, A.; Charlier, D.; Charlier, J.; Sakanyan, V.; Mett, I.; Glansdorff, N.; Gene 116, 1-6 (1992)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
argE gene encoding enzyme cloned and sequenced, M13
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
enzyme is a metalloprotein, activated by a Co2+ complex
Escherichia coli
Co2+
activity increases 8fold
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42320
-
calculated polypeptide, corresponding to AO nucleotide sequence
Escherichia coli
43000
-
gel filtration
Escherichia coli
100000
-
native enzyme, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N2-acety-L-ornithine + H2O
Escherichia coli
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P23908
K-12
-
Escherichia coli
P23908
160-37
-
Escherichia coli
P23908
ATCC9637
-
Purification (Commentary)
Purification (Commentary)
Organism
550-fold
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-acetyl-DL-methionine + H2O
-
171961
Escherichia coli
methionine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171961
Escherichia coli
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171961
Escherichia coli
L-ornithine + acetate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 43000, SDS-PAGE
Escherichia coli
Synonyms
Synonyms
Commentary
Organism
E. coli AO
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
argE gene encoding enzyme cloned and sequenced, M13
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
enzyme is a metalloprotein, activated by a Co2+ complex
Escherichia coli
Co2+
activity increases 8fold
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42320
-
calculated polypeptide, corresponding to AO nucleotide sequence
Escherichia coli
43000
-
gel filtration
Escherichia coli
100000
-
native enzyme, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N2-acety-L-ornithine + H2O
Escherichia coli
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
550-fold
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-acetyl-DL-methionine + H2O
-
171961
Escherichia coli
methionine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171961
Escherichia coli
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171961
Escherichia coli
L-ornithine + acetate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 43000, SDS-PAGE
Escherichia coli
Other publictions for EC 3.5.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755033
Molesini
The Arabidopsis N-acetylornit ...
Arabidopsis thaliana
Plant Cell Physiol.
58
130-144
2017
-
-
-
-
-
-
-
1
-
-
-
1
-
10
-
-
-
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-
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2
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3
-
-
-
1
-
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-
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-
1
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-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
1
1
734884
Molesini
Involvement of the putative N- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant Cell Physiol.
56
1084-1096
2015
-
-
1
-
1
-
-
-
-
-
-
2
-
12
-
-
-
-
-
9
-
-
2
-
4
-
-
-
-
-
-
-
-
-
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-
-
-
1
-
-
1
-
-
-
-
-
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-
-
2
-
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-
-
9
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
754657
Hernandez
Genetic and biochemical chara ...
Sinorhizobium meliloti
Microbiology
161
1671-1682
2015
-
-
1
-
-
-
1
1
-
2
-
1
-
12
-
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1
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-
-
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1
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3
1
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1
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1
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-
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-
1
-
1
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2
-
1
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1
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-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
733082
Hlavacek
Mono-N-acyl-2,6-diaminopimelic ...
Escherichia coli
Anal. Biochem.
467
4-13
2014
-
-
-
-
-
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
4
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
735279
McGregor
Identification of a histidine ...
Escherichia coli
SpringerPlus
2
482
2013
-
-
1
-
4
-
-
-
-
2
-
-
-
5
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
2
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
720166
Tao
Structural characterization of ...
Escherichia coli
J. Inorg. Biochem.
111
157-163
2012
-
-
-
-
-
-
-
2
-
-
1
-
-
6
-
-
1
-
-
-
-
-
1
1
1
-
-
-
2
-
-
-
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-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
710829
Hlavacek
Inhibitors of N(alpha)-acetyl- ...
Bacillus subtilis, Bacillus subtilis 168
Amino Acids
38
1155-1164
2010
-
-
-
-
-
-
19
-
-
-
-
-
-
98
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
18
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687815
McGregor
Characterization of the cataly ...
Escherichia coli
J. Biol. Inorg. Chem.
12
603-613
2007
-
-
-
-
-
-
1
2
-
1
1
-
-
8
-
-
1
-
-
-
-
-
1
-
2
-
-
1
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
2
-
1
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
669046
McGregor
argE-encoded N-acetyl-L-ornith ...
Escherichia coli, Escherichia coli BL21 (DE3)
J. Am. Chem. Soc.
127
14100-14107
2005
-
1
-
-
-
-
-
2
-
3
3
-
-
15
-
-
1
-
-
-
1
-
2
2
2
-
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
3
3
-
-
-
-
1
-
-
1
-
2
2
-
1
1
2
-
-
-
-
-
-
-
-
-
-
171963
Xu
Evolution of arginine biosynth ...
Escherichia coli, Moritella profunda, Moritella abyssi, Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Bacteriol.
182
1609-1615
2000
-
-
2
-
-
-
-
-
-
-
-
5
-
32
-
-
-
-
-
-
-
-
9
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
171964
Javid-Majd
Mechanistic analysis of the ar ...
Escherichia coli, Escherichia coli 160-37, Escherichia coli ATCC 9637
Biochemistry
39
1285-1293
2000
1
-
1
-
-
-
5
15
-
4
3
1
-
16
-
-
1
-
-
-
-
-
23
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
5
-
15
-
4
3
1
-
-
-
1
-
-
-
-
23
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
171961
Boyen
Acetylornithine deacetylase, s ...
Escherichia coli
Gene
116
1-6
1992
-
-
1
-
-
-
-
-
-
2
3
1
-
8
-
-
1
-
-
-
-
-
3
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
3
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
171962
Meinnel
Structural and biochemical cha ...
Escherichia coli, Saccharomyces sp., Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Bacteriol.
174
2323-2331
1992
-
-
1
-
-
-
-
1
-
-
5
4
-
21
-
-
1
-
-
-
1
-
15
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
5
4
-
-
-
1
-
-
1
-
15
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725629
Van de Casteele
-
Pathways of arginine biosynthe ...
Saccharolobus solfataricus, Saccharolobus solfataricus P1
J. Gen. Microbiol.
136
1177-1183
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
2
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
171960
Vogel
Acetylornithinase of Escherich ...
Aerobacter sp., Escherichia coli, Erwinia sp., Klebsiella sp., Proteus sp., Salmonella sp., Serratia sp., Shigella sp., Escherichia coli B / ATCC 11303, Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Biol. Chem.
218
97-106
1956
1
-
-
-
-
-
5
-
-
2
-
11
-
199
-
-
1
-
-
-
1
-
33
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
2
-
11
-
-
-
1
-
-
1
-
33
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-