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Literature summary for 3.5.1.14 extracted from

  • Wakayama, M.; Shiiba, E.; Sakai, K.; Moriguchi, M.
    Purification and characterization of L-aminoacylase from Pseudomonas maltophila B1 (1998), J. Ferment. Bioeng., 85, 278-282.
No PubMed abstract available
Search for more literature for 3.5.1.14

Application

Application Comment Organism
synthesis
-
 Sus scrofa
synthesis
-
 Aspergillus oryzae
synthesis thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates Bacillus sp. (in: Bacteria)
synthesis thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Stenotrophomonas maltophilia
1,10-phenanthroline
-
 Sus scrofa
2,3-Butanedione
-
 Stenotrophomonas maltophilia
diethylpyrocarbonate
-
 Stenotrophomonas maltophilia
DL-lactate
-
 Aspergillus oryzae
DL-lactate
-
 Stenotrophomonas maltophilia
DL-lactate
-
 Sus scrofa
glyoxylate
-
 Stenotrophomonas maltophilia
phenylboronic acid
-
 Stenotrophomonas maltophilia
Phenylglyoxal
-
 Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.32
-
 N-acetyl-L-alanine
-
 Stenotrophomonas maltophilia
12.7
-
 N-acetyl-DL-aspartate
-
 Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Stenotrophomonas maltophilia
Co2+ enzyme strongly activated by Co2+ ions Sus scrofa
Co2+ enzyme strongly activated by Co2+ ions Bacillus sp. (in: Bacteria)
Co2+ enzyme strongly activated by Co2+ ions Aspergillus oryzae
Co2+ enzyme strongly activated by Co2+ ions Stenotrophomonas maltophilia
Zn2+ enzyme contains Zn as prosthetic metal Sus scrofa
Zn2+ enzyme contains Zn as prosthetic metal Bacillus sp. (in: Bacteria)
Zn2+ enzyme contains Zn as prosthetic metal Aspergillus oryzae
Zn2+ enzyme contains Zn as prosthetic metal Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, denaturated intestinal mucosa enzyme, SDS-PAGE Sus scrofa
50000
-
 2 * 50000, native enzyme SDS-PAGE Stenotrophomonas maltophilia
108000
-
 gel filtration, native enzyme Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Bacillus sp. (in: Bacteria)
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Stenotrophomonas maltophilia
-
 L-methionine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Stenotrophomonas maltophilia
-
 gram-negative, motile, nonsporeforming rod-shaped bacterium, B1
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-aspartate + H2O
-
 Bacillus sp. (in: Bacteria) acetate + DL-aspartate
-
 ?
N-acetyl-DL-aspartate + H2O
-
 Stenotrophomonas maltophilia acetate + DL-aspartate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Bacillus sp. (in: Bacteria) DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Stenotrophomonas maltophilia DL-methionine + acetate
-
 ?
N-acetyl-DL-serine + H2O
-
 Stenotrophomonas maltophilia acetate + DL-serine
-
 ?
N-acetyl-glycine + H2O
-
 Bacillus sp. (in: Bacteria) acetate + glycine
-
 ?
N-acetyl-glycine + H2O
-
 Aspergillus oryzae acetate + glycine
-
 ?
N-acetyl-glycine + H2O
-
 Stenotrophomonas maltophilia acetate + glycine
-
 ?
N-acetyl-L-alanine + H2O
-
 Sus scrofa L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Bacillus sp. (in: Bacteria) L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Stenotrophomonas maltophilia L-alanine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Sus scrofa L-glutamine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Aspergillus oryzae L-glutamine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Stenotrophomonas maltophilia L-glutamine + acetate
-
 ?
N-acetyl-L-leucine + H2O
-
 Sus scrofa acetate + L-leucine
-
 ?
N-acetyl-L-leucine + H2O
-
 Bacillus sp. (in: Bacteria) acetate + L-leucine
-
 ?
N-acetyl-L-leucine + H2O
-
 Stenotrophomonas maltophilia acetate + L-leucine
-
 ?
N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Bacillus sp. (in: Bacteria) L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Stenotrophomonas maltophilia L-methionine + acetate
-
 ?
N-acetyl-L-phenylalanine + H2O
-
 Sus scrofa L-phenylalanine + acetate
-
 ?
N-acetyl-L-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) L-phenylalanine + acetate
-
 ?
N-acetyl-L-phenylalanine + H2O
-
 Stenotrophomonas maltophilia L-phenylalanine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Sus scrofa L-valine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Bacillus sp. (in: Bacteria) L-valine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Stenotrophomonas maltophilia L-valine + acetate
-
 ?
N-chloroacetyl-DL-phenylalanine + H2O
-
 Sus scrofa chloroacetate + phenylalanine
-
 ?
N-chloroacetyl-DL-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) chloroacetate + phenylalanine
-
 ?
N-chloroacetyl-DL-phenylalanine + H2O
-
 Aspergillus oryzae chloroacetate + phenylalanine
-
 ?
N-chloroacetyl-DL-phenylalanine + H2O
-
 Stenotrophomonas maltophilia chloroacetate + phenylalanine
-
 ?
N-chloroacetyl-DL-valine + H2O
-
 Sus scrofa chloroacetate + DL-valine
-
 ?
N-chloroacetyl-DL-valine + H2O
-
 Bacillus sp. (in: Bacteria) chloroacetate + DL-valine
-
 ?
N-chloroacetyl-DL-valine + H2O
-
 Stenotrophomonas maltophilia chloroacetate + DL-valine
-
 ?
N-formyl-L-methionine + H2O
-
 Stenotrophomonas maltophilia formate + L-methionine
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Aspergillus oryzae
dimer 2 * 45000, denaturated intestinal mucosa enzyme, SDS-PAGE Sus scrofa
dimer 2 * 50000, native enzyme SDS-PAGE Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sus scrofa
8
-
-
 Stenotrophomonas maltophilia

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Stenotrophomonas maltophilia

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 stable in wide pH-range, 5-8 at 30°C and 7-7.5 at 55°C Stenotrophomonas maltophilia