Application | Comment | Organism |
---|---|---|
synthesis | - |
Sus scrofa |
synthesis | - |
Aspergillus oryzae |
synthesis | thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
genomic DNA fragment encoding aminoacylase cloned, sequenced and expressed in Escherichia coli | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enzyme strongly activated by Co2+ ions | Sus scrofa | |
Co2+ | enzyme strongly activated by Co2+ ions | Bacillus sp. (in: Bacteria) | |
Co2+ | enzyme strongly activated by Co2+ ions | Aspergillus oryzae | |
Mn2+ | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
168000 | - |
gel filtration, low glutaraldehyde concentration, native tetrameric form | Bacillus sp. (in: Bacteria) |
180000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-methionine | Homo sapiens | - |
L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | Sus scrofa | - |
L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | Bacillus sp. (in: Bacteria) | - |
L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | Aspergillus oryzae | - |
L-methionine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
stearothermophilus | - |
Homo sapiens | - |
human | - |
Sus scrofa | - |
pig | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, expressed in Escherichia coli | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-DL-phenylalanine + H2O | - |
Bacillus sp. (in: Bacteria) | benzoate + DL-phenylalanine | - |
? | |
glycyl-L-methionine + H2O | - |
Bacillus sp. (in: Bacteria) | glycine + L-methionine | - |
? | |
glycyl-L-tyrosine + H2O | - |
Bacillus sp. (in: Bacteria) | glycine + L-tyrosine | - |
? | |
L-methionylglycine + H2O | - |
Bacillus sp. (in: Bacteria) | glycine + L-methionine | - |
? | |
N-acetyl-DL-aspartate + H2O | - |
Bacillus sp. (in: Bacteria) | acetate + DL-aspartate | - |
? | |
N-acetyl-DL-methionine + H2O | - |
Homo sapiens | DL-methionine + acetate | - |
? | |
N-acetyl-DL-methionine + H2O | - |
Sus scrofa | DL-methionine + acetate | - |
? | |
N-acetyl-DL-methionine + H2O | - |
Bacillus sp. (in: Bacteria) | DL-methionine + acetate | - |
? | |
N-acetyl-DL-methionine + H2O | - |
Aspergillus oryzae | DL-methionine + acetate | - |
? | |
N-acetyl-glycine + H2O | - |
Bacillus sp. (in: Bacteria) | acetate + glycine | - |
? | |
N-acetyl-glycine + H2O | - |
Aspergillus oryzae | acetate + glycine | - |
? | |
N-acetyl-L-alanine + H2O | - |
Sus scrofa | L-alanine + acetate | - |
? | |
N-acetyl-L-alanine + H2O | - |
Bacillus sp. (in: Bacteria) | L-alanine + acetate | - |
? | |
N-acetyl-L-alanine + H2O | - |
Aspergillus oryzae | L-alanine + acetate | - |
? | |
N-acetyl-L-arginine + H2O | - |
Sus scrofa | acetate + L-arginine | - |
? | |
N-acetyl-L-glutamine + H2O | - |
Sus scrofa | L-glutamine + acetate | - |
? | |
N-acetyl-L-glutamine + H2O | - |
Bacillus sp. (in: Bacteria) | L-glutamine + acetate | - |
? | |
N-acetyl-L-glutamine + H2O | - |
Aspergillus oryzae | L-glutamine + acetate | - |
? | |
N-acetyl-L-histidine + H2O | - |
Sus scrofa | acetate + L-histidine | - |
? | |
N-acetyl-L-leucine + H2O | - |
Sus scrofa | acetate + L-leucine | - |
? | |
N-acetyl-L-leucine + H2O | - |
Bacillus sp. (in: Bacteria) | acetate + L-leucine | - |
? | |
N-acetyl-L-methionine | - |
Homo sapiens | L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | - |
Sus scrofa | L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | - |
Bacillus sp. (in: Bacteria) | L-methionine + acetate | - |
? | |
N-acetyl-L-methionine | - |
Aspergillus oryzae | L-methionine + acetate | - |
? | |
N-acetyl-L-ornithine + H2O | - |
Bacillus sp. (in: Bacteria) | acetate + L-ornithine | - |
? | |
N-acetyl-L-phenylalanine + H2O | - |
Sus scrofa | L-phenylalanine + acetate | - |
? | |
N-acetyl-L-phenylalanine + H2O | - |
Bacillus sp. (in: Bacteria) | L-phenylalanine + acetate | - |
? | |
N-acetyl-L-tryptophan + H2O | - |
Sus scrofa | acetate + L-tryptophan | - |
? | |
N-acetyl-L-tyrosine + H2O | - |
Bacillus sp. (in: Bacteria) | tyrosine + acetate | - |
? | |
N-acetyl-L-valine + H2O | - |
Sus scrofa | L-valine + acetate | - |
? | |
N-acetyl-L-valine + H2O | - |
Bacillus sp. (in: Bacteria) | L-valine + acetate | - |
? | |
N-chloroacetyl-DL-phenylalanine + H2O | - |
Sus scrofa | chloroacetate + phenylalanine | - |
? | |
N-chloroacetyl-DL-phenylalanine + H2O | - |
Bacillus sp. (in: Bacteria) | chloroacetate + phenylalanine | - |
? | |
N-chloroacetyl-DL-phenylalanine + H2O | - |
Aspergillus oryzae | chloroacetate + phenylalanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus sp. (in: Bacteria) |