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Literature summary for 3.5.1.137 extracted from
- Cloning and expression of the carbaryl hydrolase gene mcbA and the identification of a key amino acid necessary for carbaryl hydrolysis (2018), J. Hazard. Mater., 344, 1126-1135 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. XWY-1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H451A | the mutant shows wild type activity | Pseudomonas sp. XWY-1 |
| H464A | the mutant shows wild type activity | Pseudomonas sp. XWY-1 |
| H467A | the mutant shows 32.68% activity compared to the wild type enzyme | Pseudomonas sp. XWY-1 |
| H467A/H477A | the mutant shows 7.28% activity compared to the wild type enzyme | Pseudomonas sp. XWY-1 |
| H477A | the mutant shows 78.28% activity compared to the wild type enzyme | Pseudomonas sp. XWY-1 |
| H504A | the mutant shows 1.34% activity compared to the wild type enzyme | Pseudomonas sp. XWY-1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 41.08% residual activity at 1 mM | Pseudomonas sp. XWY-1 |  |
| Hg2+ | 37.67% residual activity at 1 mM | Pseudomonas sp. XWY-1 | |
| additional information | EDTA has no effect on the enzyme activity | Pseudomonas sp. XWY-1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07767 | - |
Carbaryl | at pH 7.0 and 40°C | Pseudomonas sp. XWY-1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 122.13% activity at 1 mM | Pseudomonas sp. XWY-1 | |
| Co2+ | 158.9% activity at 1 mM | Pseudomonas sp. XWY-1 | |
| Fe2+ | 117.99% activity at 1 mM | Pseudomonas sp. XWY-1 | |
| additional information | Mn2+ has no effect on the enzyme activity | Pseudomonas sp. XWY-1 | |
| Ni2+ | 167.9% activity at 1 mM | Pseudomonas sp. XWY-1 | |
| Zn2+ | 153.08% activity at 1 mM | Pseudomonas sp. XWY-1 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. XWY-1 | A0A1S6TLL2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-bind resin column chromatography | Pseudomonas sp. XWY-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Pseudomonas sp. XWY-1 | 4-nitrophenol + acetate | - |
? | |
| aldicarb + H2O | low activity | Pseudomonas sp. XWY-1 | ? | - |
? | |
| carbaryl + H2O | best substrate | Pseudomonas sp. XWY-1 | 1-naphthol + methylamine + CO2 | - |
? | |
| carbofuran + H2O | - |
Pseudomonas sp. XWY-1 | ? | - |
? | |
| fenobucarb + H2O | - |
Pseudomonas sp. XWY-1 | ? | - |
? | |
| isoprocarb + H2O | - |
Pseudomonas sp. XWY-1 | ? | - |
? | |
| additional information | the enzyme cannot hydrolyze N-acetyl 4-aminophenol, methomyl and oxamyl | Pseudomonas sp. XWY-1 | ? | - |
- |
|
| propoxur + H2O | - |
Pseudomonas sp. XWY-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88000, SDS-PAGE | Pseudomonas sp. XWY-1 |
| ? | x * 87400, calculated from amino acid sequence | Pseudomonas sp. XWY-1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbaryl hydrolase | - |
Pseudomonas sp. XWY-1 |
| mcbA | - |
Pseudomonas sp. XWY-1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas sp. XWY-1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | more than 60% activity between 30 and 50°C | Pseudomonas sp. XWY-1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | the enzyme retains more than 80% activity after 6 h incubation at temperatures ranging from 4 to 30ºC. However, nearly 80% of the enzyme activity is lost when the temperature is higher than 50ºC for 6 h | Pseudomonas sp. XWY-1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.12 | - |
Carbaryl | at pH 7.0 and 40°C | Pseudomonas sp. XWY-1 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas sp. XWY-1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | the enzyme retains more than 80% activity after incubation at pH 6.0-8.0 | Pseudomonas sp. XWY-1 |
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