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Literature summary for 3.5.1.137 extracted from
- Purification and characterization of a novel carbaryl hydrolase from Aspergillus niger PY168 (2003), FEMS Microbiol. Lett., 228, 39-44 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-chloromercuribenzoate | 21% residual activity at 1 mM | Aspergillus niger | |
| Ag+ | complete inhibition at 1 mM | Aspergillus niger |  |
| Cu2+ | 91% residual activity at 1 mM | Aspergillus niger | |
| diisopropylfluorophosphate | 33.6% residual activity at 1 mM | Aspergillus niger | |
| Hg2+ | complete inhibition at 1 mM | Aspergillus niger | |
| iodoacetic acid | 13% residual activity at 1 mM | Aspergillus niger | |
| Mg2+ | 93% residual activity at 1 mM | Aspergillus niger | |
| Mn2+ | 96% residual activity at 1 mM | Aspergillus niger | |
| additional information | not inhibited by EDTA and phenanthroline | Aspergillus niger | |
| phenylmethylsulfonyl fluoride | 24.6% residual activity at 1 mM | Aspergillus niger | |
| Zn2+ | 95% residual activity at 1 mM | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
Carbaryl | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.036 | - |
4-nitrophenyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.039 | - |
xylylcarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.041 | - |
metolcarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.053 | - |
1-naphthyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.067 | - |
carbofuran | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.093 | - |
3,5-dimethylphenyl N-methylcarbamate | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.387 | - |
propoxur | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.551 | - |
isoprocarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 0.587 | - |
fenobucarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 1.038 | - |
Aldicarb | at pH 7.0 and 30°C | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger PY168 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate precipitation, ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Sephacryl 200 gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
cell extract, at pH 7.0 and 30°C | Aspergillus niger |
| 968 | - |
after 262fold purification, at pH 7.0 and 30°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | 73% activity compared to carbaryl | Aspergillus niger | 1-naphthol + acetate | - |
? | |
| 1-naphthyl acetate + H2O | 73% activity compared to carbaryl | Aspergillus niger PY168 | 1-naphthol + acetate | - |
? | |
| 3,5-dimethylphenyl N-methylcarbamate + H2O | 91% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
| 3,5-dimethylphenyl N-methylcarbamate + H2O | 91% activity compared to carbaryl | Aspergillus niger PY168 | ? + methylamine + CO2 | - |
? | |
| 4-nitrophenyl acetate + H2O | 77% activity compared to carbaryl | Aspergillus niger | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | 77% activity compared to carbaryl | Aspergillus niger PY168 | 4-nitrophenol + acetate | - |
? | |
| aldicarb + H2O | 18% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
| carbaryl + H2O | preferred substrate, 100% activity | Aspergillus niger | 1-naphthol + methylamine + CO2 | - |
? | |
| carbaryl + H2O | preferred substrate, 100% activity | Aspergillus niger PY168 | 1-naphthol + methylamine + CO2 | - |
? | |
| carbofuran + H2O | 83% activity compared to carbaryl | Aspergillus niger | carbofuran phenol + methylamine + CO2 | - |
? | |
| fenobucarb + H2O | 32% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
| isoprocarb + H2O | 43% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
| metolcarb + H2O | 87% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
| additional information | hydrolase activity is undetectable with chloropropham, S-ethyl dipropylthiocarbamate, and parathion | Aspergillus niger | ? | - |
- |
|
| additional information | hydrolase activity is undetectable with chloropropham, S-ethyl dipropylthiocarbamate, and parathion | Aspergillus niger PY168 | ? | - |
- |
|
| propoxur + H2O | 54% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
| xylylcarb + H2O | 93% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbaryl hydrolase | - |
Aspergillus niger |
| cehA | - |
Aspergillus niger |
| cfdJ | - |
Aspergillus niger |
| mcbA | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | more than 50% activity between 30 and 55 C. The enzyme has 56% of its full activity at 55 C and is completely inactivated at 65 C | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 548 | - |
Aldicarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 613 | - |
fenobucarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 670 | - |
isoprocarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 717 | - |
propoxur | at pH 7.0 and 30°C | Aspergillus niger | |
| 906 | - |
1-naphthyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
| 1070 | - |
4-nitrophenyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
| 1282 | - |
carbofuran | at pH 7.0 and 30°C | Aspergillus niger | |
| 1369 | - |
metolcarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 1581 | - |
3,5-dimethylphenyl N-methylcarbamate | at pH 7.0 and 30°C | Aspergillus niger | |
| 1633 | - |
xylylcarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 1724 | - |
Carbaryl | at pH 7.0 and 30°C | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | more than 75% activity between pH 5.5 and 9.0 | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | the enzyme remains stable at pH 6.0 and 8.0 with more than 90% activity remaining after 1 h | Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectric focusing | - |
4.6 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 530 | - |
Aldicarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 1040 | - |
fenobucarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 1210 | - |
isoprocarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 1850 | - |
propoxur | at pH 7.0 and 30°C | Aspergillus niger | |
| 17000 | - |
3,5-dimethylphenyl N-methylcarbamate | at pH 7.0 and 30°C | Aspergillus niger | |
| 17100 | - |
1-naphthyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
| 19300 | - |
carbofuran | at pH 7.0 and 30°C | Aspergillus niger | |
| 29700 | - |
4-nitrophenyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
| 33400 | - |
metolcarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 41800 | - |
xylylcarb | at pH 7.0 and 30°C | Aspergillus niger | |
| 144000 | - |
Carbaryl | at pH 7.0 and 30°C | Aspergillus niger | |
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