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Literature summary for 3.5.1.137 extracted from

  • Ke, Z.; Zhu, Q.; Jiang, W.; Zhou, Y.; Zhang, M.; Jiang, M.; Hong, Q.
    Heterologous expression and exploration of the enzymatic properties of the carbaryl hydrolase CarH from a newly isolated carbaryl-degrading strain (2021), Ecotoxicol. Environ. Saf., 224, 112666 .
    View publication on PubMed
Search for more literature for 3.5.1.137

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Agrobacterium sp. XWY-2

Protein Variants

Protein Variants Comment Organism
D437A inactive Agrobacterium sp. XWY-2
H341A inactive Agrobacterium sp. XWY-2
H343A inactive Agrobacterium sp. XWY-2
H346A inactive Agrobacterium sp. XWY-2
H416A inactive Agrobacterium sp. XWY-2

Inhibitors

Inhibitors Comment Organism Structure
EDTA strongly inhibited by 1 mM EDTA. The activity can be restored after supplementing with Mn2+, Co2+, Mg2+, Ca2+ and Ni2+ Agrobacterium sp. XWY-2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03801
-
 Carbaryl at pH 8.0 and 30°C Agrobacterium sp. XWY-2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-nitrophenyl acetate + H2O Agrobacterium sp. XWY-2
-
 4-nitrophenol + acetate
-
 ?
aldicarb + H2O Agrobacterium sp. XWY-2 34.74% activity compared to carbaryl ?
-
 ?
carbaryl + H2O Agrobacterium sp. XWY-2 100% activity 1-naphthol + methylamine + CO2
-
 ?
carbofuran + H2O Agrobacterium sp. XWY-2 51.24% activity compared to carbaryl 7-phenol + methylamine + CO2
-
 ?
fenobucarb + H2O Agrobacterium sp. XWY-2 69.84% activity compared to carbaryl ?
-
 ?
isoprocarb + H2O Agrobacterium sp. XWY-2 i.e. 2-isopropylphenyl N-methylcarbamate, 2% activity compared to carbaryl ?
-
 ?
additional information Agrobacterium sp. XWY-2 the enzyme does not hydrolyze N-acetyl-4-aminophenol or methomyl ?
-
-
oxamyl + H2O Agrobacterium sp. XWY-2 31.25% activity compared to carbaryl ?
-
 ?
propoxur + H2O Agrobacterium sp. XWY-2 66.01% activity compared to carbaryl ?
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium sp. XWY-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Agrobacterium sp. XWY-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Agrobacterium sp. XWY-2 4-nitrophenol + acetate
-
 ?
aldicarb + H2O 34.74% activity compared to carbaryl Agrobacterium sp. XWY-2 ?
-
 ?
carbaryl + H2O 100% activity Agrobacterium sp. XWY-2 1-naphthol + methylamine + CO2
-
 ?
carbofuran + H2O 51.24% activity compared to carbaryl Agrobacterium sp. XWY-2 7-phenol + methylamine + CO2
-
 ?
fenobucarb + H2O 69.84% activity compared to carbaryl Agrobacterium sp. XWY-2 ?
-
 ?
isoprocarb + H2O i.e. 2-isopropylphenyl N-methylcarbamate, 2% activity compared to carbaryl Agrobacterium sp. XWY-2 ?
-
 ?
additional information the enzyme does not hydrolyze N-acetyl-4-aminophenol or methomyl Agrobacterium sp. XWY-2 ?
-
-
oxamyl + H2O 31.25% activity compared to carbaryl Agrobacterium sp. XWY-2 ?
-
 ?
propoxur + H2O 66.01% activity compared to carbaryl Agrobacterium sp. XWY-2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * about 75000, SDS-PAGE Agrobacterium sp. XWY-2

Synonyms

Synonyms Comment Organism
carbaryl hydrolase
-
 Agrobacterium sp. XWY-2
CarH
-
 Agrobacterium sp. XWY-2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Agrobacterium sp. XWY-2

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 more than 50% activity between 20 and 40°C Agrobacterium sp. XWY-2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 40 the activity of the enzyme remains above 70% after incubation at 4-40°C for 2 h. When the enzyme is incubated above 60°Cfor 2 h, it loses about 95% of its activity Agrobacterium sp. XWY-2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
 Carbaryl at pH 8.0 and 30°C Agrobacterium sp. XWY-2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Agrobacterium sp. XWY-2

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 at pH values ranging from 6.0 to 8.0, the activity of the enzyme remains above 80% for 2 h Agrobacterium sp. XWY-2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.7
-
 Carbaryl at pH 8.0 and 30°C Agrobacterium sp. XWY-2