General Stability | Organism |
---|---|
the enzyme is stable in Triton X-100 and other detergents such as CHAPS | Pseudomonas sp. 50432 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | the enzyme shows 10% activity in the presence of 0.1% (v/v) 2-mercaptoethanol. Higher concentrations completely inactivate the enzyme. However, the activity of the enzyme is recovered when 2-mercaptoethanol was removed by dialysis | Pseudomonas sp. 50432 | |
dithiothreitol | the enzyme shows 10% activity in the presence of 0.1% (v/v) dithiothreitol. Higher concentrations completely inactivate the enzyme | Pseudomonas sp. 50432 | |
additional information | EDTA does not affect the enzyme activity | Pseudomonas sp. 50432 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
Carbaryl | at pH 7.0 and 25°C | Pseudomonas sp. 50432 | |
0.016 | - |
carbofuran | at pH 7.0 and 25°C | Pseudomonas sp. 50432 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pseudomonas sp. 50432 | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | cations do not affect the enzyme activity | Pseudomonas sp. 50432 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
gel filtration | Pseudomonas sp. 50432 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. 50432 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, and Macro-prep tert-butyl column chromatography | Pseudomonas sp. 50432 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
crude cell extract, at pH 7.0 and 25°C | Pseudomonas sp. 50432 |
1016 | - |
after 2032fold purification, at pH 7.0 and 25°C | Pseudomonas sp. 50432 |
Storage Stability | Organism |
---|---|
-20 or -70°C, purified enzyme in 10 mM K2HPO4 (pH 7.5) in the presence of 20% (v/v) glycerol, 1 month, no loss of activity | Pseudomonas sp. 50432 |
4°C, purified enzyme in 10 mM K2HPO4 (pH 7.5), 1 month, 25% loss of activity | Pseudomonas sp. 50432 |
4°C, purified enzyme in 10 mM K2HPO4 (pH 7.5), 1 week, no loss of activity | Pseudomonas sp. 50432 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Pseudomonas sp. 50432 | 1-naphthol + acetate | - |
? | |
carbaryl + H2O | - |
Pseudomonas sp. 50432 | 1-naphthol + methylamine + CO2 | - |
? | |
carbofuran + H2O | - |
Pseudomonas sp. 50432 | 7-phenol + methylamine + CO2 | - |
? | |
additional information | the purified enzyme does not hydrolyze 2-nitrophenyl dimethylcarbamate | Pseudomonas sp. 50432 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 88000, SDS-PAGE | Pseudomonas sp. 50432 |
Synonyms | Comment | Organism |
---|---|---|
carbamate hydrolase | - |
Pseudomonas sp. 50432 |
carbofuran hydrolase | - |
Pseudomonas sp. 50432 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas sp. 50432 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
when heated at 60°C for 5 min, the catalytic activity of the enzyme is completely lost | Pseudomonas sp. 50432 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas sp. 50432 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. 50432 | isoelectric focusing | - |
6.9 |