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Literature summary for 3.5.1.137 extracted from

  • Jiang, W.; Zhang, C.; Gao, Q.; Zhang, M.; Qiu, J.; Yan, X.; Hong, Q.
    Carbamate C-N hydrolase gene ameH responsible for the detoxification step of methomyl degradation in Aminobacter aminovorans strain MDW-2 (2020), Appl. Environ. Microbiol., 87, e02005-20 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.137

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aminobacter aminovorans

Protein Variants

Protein Variants Comment Organism
D191A the activity of the mutant enzyme is completely abolished Aminobacter aminovorans
E207A the activity of the mutant enzyme is completely abolished Aminobacter aminovorans
G162A the activity of the mutant enzyme is completely abolished Aminobacter aminovorans
H193A the mutant retains 10% of hydrolysis activity against methomyl as compared to the wild type enzyme Aminobacter aminovorans
N164A the activity of the mutant enzyme is completely abolished Aminobacter aminovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0875
-
 methomyl at pH 7.5 and 30°C Aminobacter aminovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Aminobacter aminovorans

Organism

Organism UniProt Comment Textmining
Aminobacter aminovorans
-
-
-
Aminobacter aminovorans MDW-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex-200 gel filtration Aminobacter aminovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O
-
 Aminobacter aminovorans ?
-
 ?
acetamide + H2O
-
 Aminobacter aminovorans MDW-2 ?
-
 ?
formamide + H2O
-
 Aminobacter aminovorans ?
-
 ?
formamide + H2O
-
 Aminobacter aminovorans MDW-2 ?
-
 ?
methomyl + H2O
-
 Aminobacter aminovorans methyl (1E)-N-hydroxyethanimidothioate + methylamine + CO2
-
 ?
methomyl + H2O
-
 Aminobacter aminovorans MDW-2 methyl (1E)-N-hydroxyethanimidothioate + methylamine + CO2
-
 ?
additional information the enzyme shows no catalytic activity on any carbamate pesticide other than methomyl, including carbaryl, isoprocarb, fenobucarb, propoxur, aldicarb, carbofuran, and oxamyl Aminobacter aminovorans ?
-
-
additional information the enzyme shows no catalytic activity on any carbamate pesticide other than methomyl, including carbaryl, isoprocarb, fenobucarb, propoxur, aldicarb, carbofuran, and oxamyl Aminobacter aminovorans MDW-2 ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 34000, His6-tagged enzyme, SDS-PAGE Aminobacter aminovorans

Synonyms

Synonyms Comment Organism
ameH
-
 Aminobacter aminovorans
carbamate C-N hydrolase
-
 Aminobacter aminovorans
methomyl hydrolase
-
 Aminobacter aminovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aminobacter aminovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 the enzyme retains more than 80% of its activity at 55°C for 1 h. At 70°C, the residual enzyme activity falls below 10% in 1 h Aminobacter aminovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
345.2
-
 methomyl at pH 7.5 and 30°C Aminobacter aminovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Aminobacter aminovorans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 the enzyme exhibits high activity levels at a pH range of 6.5 to 9.0 Aminobacter aminovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.5 the enzyme retains 75% of its activity after storage at pH 7.0 to 8.5 for 1 h at 30°C Aminobacter aminovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3900
-
 methomyl at pH 7.5 and 30°C Aminobacter aminovorans