Cloned (Comment) | Organism |
---|---|
enzyme variant rCHDELTA(Tmd)a is expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. C5pp |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.098 | - |
Carbaryl | native enzyme, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C5pp | |
0.111 | - |
Carbaryl | native enzyme, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C7 | |
0.111 | - |
Carbaryl | recombinant enzyme rCHDELTA(Tmd)a, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C5pp |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas sp. C5pp | - |
- |
periplasm | - |
Pseudomonas sp. C7 | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
native enzyme, gel filtration | Pseudomonas sp. C5pp |
70000 | - |
native enzyme, gel filtration | Pseudomonas sp. C7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbaryl + H2O | Pseudomonas sp. C5pp | - |
1-naphthol + methylamine + CO2 | - |
? | |
carbaryl + H2O | Pseudomonas sp. C7 | - |
1-naphthol + methylamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. C5pp | A0A140DCH5 | - |
- |
Pseudomonas sp. C7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Sepharose CL-4B column chromatography, hydroxyapatite column chromatography and Sephacryl S-200HR gel filtration | Pseudomonas sp. C7 |
Q-Sepharose column chromatography, Sepharose CL-4B column chromatography, hydroxyapatite column chromatography and Sephacryl S-200HR gel filtration, and Ni-NTA column chromatography | Pseudomonas sp. C5pp |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbaryl + H2O | - |
Pseudomonas sp. C5pp | 1-naphthol + methylamine + CO2 | - |
? | |
carbaryl + H2O | - |
Pseudomonas sp. C7 | 1-naphthol + methylamine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, native enzyme, SDS-PAGE | Pseudomonas sp. C5pp |
monomer | 1 * 68000, native enzyme, SDS-PAGE | Pseudomonas sp. C7 |
monomer | 1 * 76000, recombinant enzyme rCHDELTA(Tmd)a, SDS-PAGE | Pseudomonas sp. C5pp |
Synonyms | Comment | Organism |
---|---|---|
carbaryl hydrolase | - |
Pseudomonas sp. C5pp |
carbaryl hydrolase | - |
Pseudomonas sp. C7 |
mcbA | - |
Pseudomonas sp. C5pp |
mcbA | - |
Pseudomonas sp. C7 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
81 | - |
Carbaryl | native enzyme, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C5pp | |
84 | - |
Carbaryl | native enzyme, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C7 | |
86 | - |
Carbaryl | recombinant enzyme rCHDELTA(Tmd)a, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C5pp |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
760 | - |
Carbaryl | native enzyme, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C7 | |
770 | - |
Carbaryl | recombinant enzyme rCHDELTA(Tmd)a, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C5pp | |
830 | - |
Carbaryl | native enzyme, at pH 7.5, temperature not specified in the publication | Pseudomonas sp. C5pp |