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Literature summary for 3.5.1.13 extracted from

  • Ko, H.J.; Lee, E.W.; Bang, W.G.; Lee, C.K.; Kim, K.H.; Choi, I.G.
    Molecular characterization of a novel bacterial aryl acylamidase belonging to the amidase signature enzyme family (2010), Mol. Cells, 29, 485-492.
    View publication on PubMed
Search for more literature for 3.5.1.13

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic library construction and cloning in Escherichia coli strains NM522 and DH5alpha, expression in Escherichia coli strain BL21(DE3) bacterium CSBL00001

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits 21.1% at 1 mM and 21.5% at 10 mM bacterium CSBL00001
Co2+ inhibits 14.1% at 1 mM and 17.4% at 10 mM bacterium CSBL00001
Cu2+ inhibits 13.4% at 1 mM and 45.7% at 10 mM bacterium CSBL00001
Ni2+ inhibits 26.0% at 1 mM and 43.2% at 10 mM bacterium CSBL00001
Zn2+ inhibits 40.0% at 1 mM and 75.8% at 10 mM bacterium CSBL00001

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 4-nitroacetanilide pH 9.0, 37°C bacterium CSBL00001
0.32
-
 N-(4-hydroxyphenyl)acetamide pH 9.0, 37°C bacterium CSBL00001
0.83
-
 4-chloroacetanilide pH 9.0, 37°C bacterium CSBL00001
19
-
 acetanilide pH 9.0, 37°C bacterium CSBL00001

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-acetamidophenol + H2O bacterium CSBL00001 i.e. Tylenol acetate + 4-aminophenol
-
 r
4-chloroacetanilide + H2O bacterium CSBL00001
-
 acetate + 4-chloroaniline
-
 r
4-nitroacetanilide + H2O bacterium CSBL00001
-
 acetate + 4-nitroaniline
-
 r
acetanilide + H2O bacterium CSBL00001
-
 acetate + aniline
-
 r
phenacetin + H2O bacterium CSBL00001
-
 acetate + 4-ethoxyaniline
-
 r

Organism

Organism UniProt Comment Textmining
bacterium CSBL00001 C3UWD1 a soil bacterium, might be a Pseudomonas species
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-acetamidophenol + H2O i.e. Tylenol bacterium CSBL00001 acetate + 4-aminophenol
-
 r
4-acetamidophenol + H2O i.e. Tylenol. The enzyme contains a revealed a conserved amidase signature region with a canonical catalytic triad bacterium CSBL00001 acetate + 4-aminophenol
-
 r
4-chloroacetanilide + H2O
-
 bacterium CSBL00001 acetate + 4-chloroaniline
-
 r
4-nitroacetanilide + H2O
-
 bacterium CSBL00001 acetate + 4-nitroaniline
-
 r
acetanilide + H2O
-
 bacterium CSBL00001 acetate + aniline
-
 r
phenacetin + H2O
-
 bacterium CSBL00001 acetate + 4-ethoxyaniline
-
 r
phenacetin + H2O i.e. N-(4-ethoxyphenyl)acetamide bacterium CSBL00001 acetate + 4-ethoxyaniline
-
 r

Synonyms

Synonyms Comment Organism
AAA
-
 bacterium CSBL00001
More the enzyme belongs to the amidase signature, AS, enzyme family bacterium CSBL00001

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 recombinant enzyme bacterium CSBL00001

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 40 purified recombinant enzyme, pH 9.0, the half-life of enzyme activity at 37°C is 192 h, and 90% of its activity remain after 3 h incubation at 40°C bacterium CSBL00001
45
-
 purified recombinant enzyme, 3 h, pH 9.0, stable up to bacterium CSBL00001
55
-
 purified recombinant enzyme, 3 h, pH 9.0, 50% activity remaining bacterium CSBL00001
70
-
 purified recombinant enzyme, 3 h, pH 9.0, inactivation bacterium CSBL00001

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7 reverse reaction bacterium CSBL00001
10
-
 recombinant enzyme, forward reaction bacterium CSBL00001