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Literature summary for 3.5.1.13 extracted from
- Properties of arylamidase from Aspergillus oryzae (1978), Agric. Biol. Chem., 42, 1291-1292.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Aspergillus oryzae |  |
| 2-mercaptoethanol | - |
Aspergillus oryzae | |
| 8-hydroxyquinoline | - |
Aspergillus oryzae | |
| diisopropylphosphofluoridate | complete inhibition at 1 mM , scarcely inhibited at 0.1 mM | Aspergillus oryzae | |
| EDTA | - |
Aspergillus oryzae | |
| formaldehyde | - |
Aspergillus oryzae | |
| Mn2+ | - |
Aspergillus oryzae | |
| N-bromosuccinimide | - |
Aspergillus oryzae | |
| Ni2+ | - |
Aspergillus oryzae | |
| Sodium thioglycolate | - |
Aspergillus oryzae | |
| Zn2+ | - |
Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-NH2 + H2O | - |
Aspergillus oryzae | Ala + NH3 | - |
? | |
| Asp-p-nitroanilide + H2O | - |
Aspergillus oryzae | Asp + p-nitroaniline | - |
? | |
| Gly-2-naphthylamide + H2O | - |
Aspergillus oryzae | Gly 2-naphthylamine | - |
? | |
| Gly-Phe-NH2 + H2O | - |
Aspergillus oryzae | Gly-Phe + NH3 | - |
? | |
| Gly-Pro-Leu-p-nitroanilide + H2O | - |
Aspergillus oryzae | Gly-Pro-Leu + p-nitroaniline | - |
? | |
| L-Glu-beta-naphthylamide + H2O | - |
Aspergillus oryzae | Glu + beta-naphthylamine | - |
? | |
| L-Leu p-nitroanilide + H2O | - |
Aspergillus oryzae | Leu + p-nitroaniline | - |
? | |
| L-Leu-beta-naphthylamide + H2O | - |
Aspergillus oryzae | Leu + beta-naphthylamine | - |
? | |
| Leu-Gly-NH2 + H2O | - |
Aspergillus oryzae | Leu-Gly + NH3 | - |
? | |
| Leu-NH2 + H2O | - |
Aspergillus oryzae | Leu + NH3 | - |
? | |
| Phe-NH2 + H2O | - |
Aspergillus oryzae | Phe + NH3 | - |
? | |
| Pro-NH2 + H2O | - |
Aspergillus oryzae | Pro + NH3 | - |
? | |
| Val-beta-naphthylamide + H2O | - |
Aspergillus oryzae | Val + beta-naphthylamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | hydrolysis of Leu-p-nitroanilide | Aspergillus oryzae |
| 50 | - |
hydrolysis of Leu-beta-naphthylamide | Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to | Aspergillus oryzae |
| 60 | - |
stable for at least 90 min | Aspergillus oryzae |
| 70 | - |
10 min, complete inactivation | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
hydrolysis of Phe-NH2 | Aspergillus oryzae |
| 8.5 | - |
hydrolysis of Leu-beta-naphthylamine and Leu-p-nitroanilide | Aspergillus oryzae |
| 9 | - |
hydrolysis of Ala-NH2 | Aspergillus oryzae |
| 9.5 | - |
hydrolysis of Leu-NH2 | Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | stable | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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