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Literature summary for 3.5.1.116 extracted from

  • Werner, A.K.; Medina-Escobar, N.; Zulawski, M.; Sparkes, I.A.; Cao, F.Q.; Witte, C.P.
    The ureide-degrading reactions of purine ring catabolism employ three amidohydrolases and one aminohydrolase in Arabidopsis, soybean, and rice (2013), Plant Physiol., 163, 672-681.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.116

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana benthamiana leaf epidermal cells Glycine max
expressed in Nicotiana benthamiana leaf epidermal cells Arabidopsis thaliana
expressed in Nicotiana benthamiana leaf epidermal cells Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Glycine max
0.188
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum predominant localisation Glycine max 5783
-
endoplasmic reticulum predominant localisation Arabidopsis thaliana 5783
-
endoplasmic reticulum predominant localisation Oryza sativa 5783
-
Golgi apparatus
-
 Glycine max 5794
-
Golgi apparatus
-
 Arabidopsis thaliana 5794
-
Golgi apparatus
-
 Oryza sativa 5794
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-ureidoglycolate + H2O Glycine max
-
 glyoxylate + 2 NH3 + CO2
-
 ?
(S)-ureidoglycolate + H2O Arabidopsis thaliana
-
 glyoxylate + 2 NH3 + CO2
-
 ?
(S)-ureidoglycolate + H2O Oryza sativa
-
 glyoxylate + 2 NH3 + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Glycine max
-
-
-
Oryza sativa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Glycine max
-
leaf
-
 Arabidopsis thaliana
-
leaf
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 at pH 7.0 and 37°C Glycine max
64
-
 at pH 7.0 and 37°C Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-ureidoglycolate + H2O
-
 Glycine max glyoxylate + 2 NH3 + CO2
-
 ?
(S)-ureidoglycolate + H2O
-
 Arabidopsis thaliana glyoxylate + 2 NH3 + CO2
-
 ?
(S)-ureidoglycolate + H2O
-
 Oryza sativa glyoxylate + 2 NH3 + CO2
-
 ?

Synonyms

Synonyms Comment Organism
UAH
-
 Glycine max
UAH
-
 Arabidopsis thaliana
UAH
-
 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Glycine max
55
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
295
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Oryza sativa
430
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Glycine max