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Literature summary for 3.5.1.111 extracted from

  • Cobzaru, C.; Ganas, P.; Mihasan, M.; Schleberger, P.; Brandsch, R.
    Homologous gene clusters of nicotine catabolism, including a new omega-amidase for alpha-ketoglutaramate, in species of three genera of Gram-positive bacteria (2011), Res. Microbiol., 162, 285-291.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3
-
2-Oxoglutaramate pH 7.1, 30 °C Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32300
-
calculated from cDNA Paenarthrobacter nicotinovorans
137000
-
gel filtration Paenarthrobacter nicotinovorans

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans Q93NG1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutaramate + H2O
-
Paenarthrobacter nicotinovorans ?
-
?
L-glutamic acid + H2O poor substrate Paenarthrobacter nicotinovorans ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 32300, gel filtration Paenarthrobacter nicotinovorans

Synonyms

Synonyms Comment Organism
omega-amidase
-
Paenarthrobacter nicotinovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Paenarthrobacter nicotinovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
194
-
2-Oxoglutaramate pH 7.1, 30 °C Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
assay at Paenarthrobacter nicotinovorans