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Literature summary for 3.5.1.11 extracted from

  • Avinash, V.S.; Panigrahi, P.; Chand, D.; Pundle, A.; Suresh, C.G.; Ramasamy, S.
    Structural analysis of a penicillin V acylase from Pectobacterium atrosepticum confirms the importance of two Trp residues for activity and specificity (2016), J. Struct. Biol., 193, 85-94 .
    View publication on PubMed
Search for more literature for 3.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
the pva gene is cloned into a pET28b plasmid vector between NcoI and XhoI restriction sites. The PVA enzyme is expressed in Escherichia coli BL21 star cells Pectobacterium atrosepticum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 22°C, crystal structure at 2.5 A resolution Pectobacterium atrosepticum

Protein Variants

Protein Variants Comment Organism
C1S no activity Pectobacterium atrosepticum
D22E the specific activity is 10.9% compared to wild-type enzyme. Protein expression yields is 34 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
D22N the specific activity is 5.1% compared to wild-type enzyme. Protein expression yields is 2.5 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
R19H the specific activity is 6% compared to wild-type enzyme. Protein expression yields is 121 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
R215L the specific activity is 7.4% compared to wild-type enzyme. Protein expression yields is 5.6 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
W23F the specific activity is 4% compared to wild-type enzyme. Protein expression yields is 6.9 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
W23F/W87Y the specific activity is 0.13% compared to wild-type enzyme. Protein expression yields is 3.3 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
W23I the specific activity is 0.9% compared to wild-type enzyme. Protein expression yields is 7.1 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
W23I/W87N the specific activity is 0.02% compared to wild-type enzyme. Protein expression yields is 3.6 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
W87N the specific activity is 81.2% compared to wild-type enzyme. Protein expression yields is 196.8 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum
W87Y the specific activity is 1.5% compared to wild-type enzyme. Protein expression yields is 6.7 mg/ml compared to 262 mg/ml for the native enzyme Pectobacterium atrosepticum

Organism

Organism UniProt Comment Textmining
Pectobacterium atrosepticum Q6D291
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-
Pectobacterium atrosepticum ATCC BAA-672 Q6D291
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Pectobacterium atrosepticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin V + H2O
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 Pectobacterium atrosepticum 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?
penicillin V + H2O
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 Pectobacterium atrosepticum ATCC BAA-672 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?

Synonyms

Synonyms Comment Organism
penicillin V acylase
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 Pectobacterium atrosepticum