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Literature summary for 3.5.1.11 extracted from

  • Grulich, M.; Brezovsky, J.; Stepanek, V.; Palyzova, A.; Kyslikova, E.; Kyslik, P.
    Resolution of alpha/beta-amino acids by enantioselective penicillin G acylase from Achromobacter sp. (2015), J. Mol. Catal. B, 122, 240-247 .
No PubMed abstract available
Search for more literature for 3.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
expression in Escherichia coli BL21 Achromobacter sp. CCM 4824

Organism

Organism UniProt Comment Textmining
Achromobacter sp. CCM 4824 Q3ZEF0
-
-
Escherichia coli P06875
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Achromobacter sp. CCM 4824

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-phenylacetyl-alpha-homophenylalanine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80 Escherichia coli homophenylalanine + phenylacetate
-
 ?
N-phenylacetyl-alpha-homophenylalanine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80 Achromobacter sp. CCM 4824 homophenylalanine + phenylacetate
-
 ?
N-phenylacetyl-alpha-isoleucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80 Escherichia coli isoleucine + phenylacetate
-
 ?
N-phenylacetyl-alpha-isoleucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80 Achromobacter sp. CCM 4824 isoleucine + phenylacetate
-
 ?
N-phenylacetyl-alpha-leucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80 Escherichia coli leucine + phenylacetate
-
 ?
N-phenylacetyl-alpha-leucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80 Achromobacter sp. CCM 4824 leucine + phenylacetate
-
 ?
N-phenylacetyl-alpha-phenylalanine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 90 Escherichia coli phenylalanine + phenylacetate
-
 ?
N-phenylacetyl-alpha-phenylalanine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 90 Achromobacter sp. CCM 4824 phenylalanine + phenylacetate
-
 ?
N-phenylacetyl-alpha-tert-leucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 105 Achromobacter sp. CCM 4824 tert-leucine + phenylacetate
-
 ?
N-phenylacetyl-alpha-tert-leucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 75 Escherichia coli tert-leucine + phenylacetate
-
 ?
N-phenylacetyl-beta-homoleucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 55 Escherichia coli beta-homoleucine + phenylacetate
-
 ?
N-phenylacetyl-beta-homoleucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 85 Achromobacter sp. CCM 4824 beta-homoleucine + phenylacetate
-
 ?
N-phenylacetyl-beta-leucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 60 Escherichia coli beta-leucine + phenylacetate
-
 ?
N-phenylacetyl-beta-leucine + H2O experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 68 Achromobacter sp. CCM 4824 beta-leucine + phenylacetate
-
 ?

Synonyms

Synonyms Comment Organism
penicillin G acylase
-
 Escherichia coli
penicillin G acylase
-
 Achromobacter sp. CCM 4824

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Achromobacter sp. CCM 4824

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli
8
-
 assay at Achromobacter sp. CCM 4824